Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care

0565231 - ÚMCH 2023 RIV US eng J - Journal Article
Lytvynchuk, L. - Straňák, Z. - Studenovská, Hana - Rais, David - Popelka, Štěpán - Tichotová, Lucie - Nemesh, Yaroslav - Kolesnikova, Anastasiia - Nyshchuk, Ruslan - Brymová, Anna - Ellederová, Zdeňka - Čížková, Jana - Juhásová, Jana - Juhás, Štefan - Jendelová, Pavla - Nagymihály, R. - Kozak, I. - Erceg, S. - Binder, S. - Müller, B. - Stieger, K. - Motlík, Jan - Petrovski, G. - Ardan, Taras
Subretinal implantation of RPE on a carrier in minipigs: guidelines for preoperative preparations, surgical techniques, and postoperative care.
Jove-Journal of Visualized Experiments. Roč. 2022, č. 189 (2022), č. článku e63505. ISSN 1940-087X
R&D Projects: GA ČR(CZ) GA18-04393S; GA TA ČR(CZ) TO01000107
Institutional support: RVO:61389013 ; RVO:67985904 ; RVO:68378041
Keywords : age-related macular degeneration * retinal pigment epithelium (RPE) * large eye animal model
OECD category: Polymer science; Ophthalmology (UZFG-Y); Neurosciences (including psychophysiology (UEM-P)
Impact factor: 1.2, year: 2022
Method of publishing: Limited access
https://review.jove.com/t/63505/subretinal-implantation-rpe-on-carrier-minipigs-guidelines-for

Degenerative disorders of the retina (including age-related macular degeneration), which originate primarily at or within the retinal pigmented epithelial (RPE) layer, lead to a progressive disorganization of the retinal anatomy and the deterioration of visual function. The substitution of damaged RPE cells (RPEs) with in vitro cultured RPE cells using a subretinal cell carrier has shown potential for re-establishing the anatomical structure of the outer retinal layers and is, therefore, being further studied. Here, we present the principles of a surgical technique that allows for the effective subretinal transplantation of a cell carrier with cultivated RPEs into minipigs. The surgeries were performed under general anesthesia and included a standard lens-sparing three-port pars plana vitrectomy (PPV), subretinal application of a balanced salt solution (BSS), a 2.7 mm retinotomy, implantation of a nanofibrous cell carrier into the subretinal space through an additional 3.0 mm sclerotomy, fluid-air exchange (FAX), silicone oil tamponade, and closure of all the sclerotomies. This surgical approach was used in 29 surgeries (18 animals) over the past 8 years with a success rate of 93.1%. Anatomic verification of the surgical placement was carried out using in vivo fundus imaging (fundus photography and optical coherence tomography). The recommended surgical steps for the subretinal implantation of RPEs on a carrier in minipig eyes can be used in future preclinical studies using large-eye animal models.
Permanent Link: https://hdl.handle.net/11104/0337462