Polyenic antibiotics and other antifungal compounds produced by hemolytic Streptomyces species

0565131 - BC 2023 RIV CH eng J - Journal Article
Bobek, J. - Filipová, E. - Bergman, N. - Čihák, M. - Petříček, M. - Lara, Ana Catalina - Krištůfek, Václav - Megyes, M. - Wurzer, T. - Chroňáková, Alica - Petříčková, K.
Polyenic antibiotics and other antifungal compounds produced by hemolytic Streptomyces species.
International Journal of Molecular Sciences. Roč. 23, č. 23 (2022), č. článku 15045. E-ISSN 1422-0067
R&D Projects: GA MZd(CZ) NV17-30091A; GA ČR(CZ) GA17-22572S
Grant - others:AV ČR(CZ) StrategieAV21/9; AV ČR(CZ) StrategieAV21/21
Program: StrategieAV; StrategieAV
Institutional support: RVO:60077344
Keywords : Actinomycetales * secondary metabolites * polyene antibiotics * Streptomyces * hemolysis * symbiosis * soil ecosystem
OECD category: Biochemistry and molecular biology
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1422-0067/23/23/15045

Streptomyces are of great interest in the pharmaceutical industry as they produce a plethora of secondary metabolites that act as antibacterial and antifungal agents. They may thrive on their own in the soil, or associate with other organisms, such as plants or invertebrates. Some soil-derived strains exhibit hemolytic properties when cultivated on blood agar, raising the question of whether hemolysis could be a virulence factor of the bacteria. In this work we examined hemolytic compound production in 23 β-hemolytic Streptomyces isolates, of these 12 were soil-derived, 10 were arthropodassociated, and 1 was plant-associated. An additional human-associated S. sp. TR1341 served as a control. Mass spectrometry analysis suggested synthesis of polyene molecules responsible for the hemolysis: candicidins, filipins, strevertene A, tetrafungin, and tetrin A, as well as four novel polyene compounds (denoted here as polyene A, B, C, and D) in individual liquid cultures or paired cocultures. The non-polyene antifungal compounds actiphenol and surugamide A were also identified. The findings indicate that the ability of Streptomyces to produce cytolytic compounds (here manifested by hemolysis on blood agar) is an intrinsic feature of the bacteria in the soil environment and could even serve as a virulence factor when colonizing available host organisms. Additionally, a literature review of polyenes and non-polyene hemolytic metabolites produced by Streptomyces is presented.
Permanent Link: https://hdl.handle.net/11104/0336979