Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles

0565047 - ÚEB 2023 RIV US eng J - Journal Article
Gámez-Arcas, S. - Munoz, F.J. - Ricarte-Bermejo, A. - Sánchez-López, Á. M. - Baslam, M. - Baroja-Fernández, E. - Bahaji, A. - Almagro, G. - De Diego, N. - Doležal, Karel - Novák, Ondřej - Leal-López, J. - Morcillo, R. J. L. - Castillo, A. G. - Pozueta-Romero, J.
Glucose-6-P/phosphate translocator2 mediates the phosphoglucose-isomerase1-independent response to microbial volatiles.
Plant Physiology. Roč. 190, č. 4 (2022), s. 2137-2154. ISSN 0032-0889. E-ISSN 1532-2548
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : GLUCOSE 6-PHOSPHATE/PHOSPHATE TRANSLOCATOR * AUTOCRINE MOTILITY FACTOR * PHOSPHOGLUCOSE ISOMERASE
OECD category: Biochemistry and molecular biology
Impact factor: 7.4, year: 2022
Method of publishing: Open access
https://doi.org/10.1093/plphys/kiac433

In Arabidopsis (Arabidopsis thaliana), the plastidial isoform of phosphoglucose isomerase (PGI1) mediates photosynthesis, metabolism, and development, probably due to its involvement in the synthesis of isoprenoid-derived signals in vascular tissues. Microbial volatile compounds (VCs) with molecular masses of <45 Da promote photosynthesis, growth, and starch overaccumulation in leaves through PGI1-independent mechanisms. Exposure to these compounds in leaves enhances the levels of GLUCOSE-6-PHOSPHATE/PHOSPHATE TRANSLOCATOR2 (GPT2) transcripts. We hypothesized that the PGI1-independent response to microbial volatile emissions involves GPT2 action. To test this hypothesis, we characterized the responses of wild-type (WT), GPT2-null gpt2-1, PGI1-null pgi1-2, and pgi1-2gpt2-1 plants to small fungal VCs. In addition, we characterized the responses of pgi1-2gpt2-1 plants expressing GPT2 under the control of a vascular tissue- and root tip-specific promoter to small fungal VCs. Fungal VCs promoted increases in growth, starch content, and photosynthesis in WT and gpt2-1 plants. These changes were substantially weaker in VC-exposed pgi1-2gpt2-1 plants but reverted to WT levels with vascular and root tip-specific GPT2 expression. Proteomic analyses did not detect enhanced levels of GPT2 protein in VC-exposed leaves and showed that knocking out GPT2 reduced the expression of photosynthesis-related proteins in pgi1-2 plants. Histochemical analyses of GUS activity in plants expressing GPT2-GUS under the control of the GPT2 promoter showed that GPT2 is mainly expressed in root tips and vascular tissues around hydathodes. Overall, the data indicated that the PGI1-independent response to microbial VCs involves resetting of the photosynthesis-related proteome in leaves through long-distance GPT2 action.
Permanent Link: https://hdl.handle.net/11104/0336601