Magnetron-sputtered niobium nanoparticles for molecular imaging of brain tissues through surface-assisted laser desorption/ionization mass spectrometry

0564922 - FZÚ 2023 RIV US eng J - Journal Article
Pleskunov, P. - Prysiazhnyi, V. - Nikitin, D. - Košutová, T. - Cieslar, M. - Gordeev, Ivan - Krtouš, Z. - Ali-Ogly, S. - Šomvársky, J. - Protsak, M. - Biliak, K. - Kishenina, K. - Bednařík, A. - Dopita, M. - Preisler, J. - Choukourov, A.
Magnetron-sputtered niobium nanoparticles for molecular imaging of brain tissues through surface-assisted laser desorption/ionization mass spectrometry.
ACS Applied Nano Materials. Roč. 5, č. 9 (2022), s. 12865-12875. ISSN 2574-0970
Institutional support: RVO:68378271
Keywords : magnetron sputtering * niobium * nanoparticles * biomolecules detection * biological interface * SALDI MS
OECD category: Nano-processes (applications on nano-scale)
Impact factor: 5.9, year: 2022
Method of publishing: Limited access
https://doi.org/10.1021/acsanm.2c02734

The Nb NPs are synthesized by a magnetron sputtering inert gas aggregation technique, which avoids the use of wet chemical pathways and allows the direct deposition of the NPs onto biological specimens. The NPs are partially oxidized and consist of a body-centered cubic (bcc) polycrystalline metal Nb core enclosed by an amorphous Nb2O5 shell. When seeded onto slices of the mouse brain, the Nb NPs form an effective matrix for high-resolution SALDI MSI of complex biological samples in both negative and positive ionization modes. The use of ultrahigh-resolution mass spectrometry allowed the identification of different Nb adducts and multiple phospholipid ion classes at m/z < 1000 This work qualifies Nb NPs as an efficient, accessible, and easy-to-handle matrix for SALDI MSI for the first time. In addition, it highlights the potential utilization of other, yet unexplored transition-metal NPs, motivating their future research.
Permanent Link: https://hdl.handle.net/11104/0336498