DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation

0564845 - MBÚ 2023 RIV DE eng J - Journal Article
Vergani, L. - Mapelli, F. - Folkmanová, M. - Papík, J. - Jansa, Jan - Uhlík, O. - Borin, S.
DNA stable isotope probing on soil treated by plant biostimulation and flooding revealed the bacterial communities involved in PCB degradation.
Scientific Reports. Roč. 12, č. 1 (2022), č. článku 19232. ISSN 2045-2322. E-ISSN 2045-2322
EU Projects: European Commission(CZ) 841317
Institutional support: RVO:61388971
Keywords : Polychlorinated biphenyl (PCB) * microbial PCB degraders * stable isotope probing SIP incubation * illumina MiSeq * sequencing * soil bacterial communities * Betaproteobacteria * Actinobacteria
OECD category: Microbiology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41598-022-23728-2

Polychlorinated biphenyl (PCB)-contaminated soils represent a major treat for ecosystems health. Plant biostimulation of autochthonous microbial PCB degraders is a way to restore polluted sites where traditional remediation techniques are not sustainable, though its success requires the understanding of site-specific plant-microbe interactions. In an historical PCB contaminated soil, we applied DNA stable isotope probing (SIP) using C-13-labeled 4-chlorobiphenyl (4-CB) and 16S rRNA MiSeq amplicon sequencing to determine how the structure of total and PCB-degrading bacterial populations were affected by different treatments: biostimulation with Phalaris arundinacea subjected (PhalRed) or not (Phal) to a redox cycle and the non-planted controls (Bulk and BulkRed). Phal soils hosted the most diverse community and plant biostimulation induced an enrichment of Actinobacteria. Mineralization of 4-CB in SIP microcosms varied between 10% in Bulk and 39% in PhalRed soil. The most abundant taxa deriving carbon from PCB were Betaproteobacteria and Actinobacteria. Comamonadaceae was the family most represented in Phal soils, Rhodocyclaceae and Nocardiaceae in non-planted soils. Planted soils subjected to redox cycle enriched PCB degraders affiliated to Pseudonocardiaceae, Micromonosporaceae and Nocardioidaceae. Overall, we demonstrated different responses of soil bacterial taxa to specific rhizoremediation treatments and we provided new insights into the populations active in PCB biodegradation.
Permanent Link: https://hdl.handle.net/11104/0336409