Bioactive Natural Products

0564409 - MBÚ 2023 RIV NL eng M - Monography Chapter
Palyzová, Andrea - Spížek, Jaroslav - Vítová, Milada - Řezanka, Tomáš
Biosynthesis of polyunsaturated fatty acids by metabolic engineering of yeast Yarrowia lipolytica.
Bioactive Natural Products. Amsterdam: Elsevier B.V., 2022 - (Atta-ur-Rahman), s. 197-223. ISBN 978-0-323-91099-6
Institutional support: RVO:61388971
Keywords : Yeast * Yarrowia lipolytica * Polyunsaturated fatty acids * Genetically modified * biosynthesis
OECD category: Microbiology
https://www.sciencedirect.com/science/article/abs/pii/B9780323910996000074

Metabolic engineering describes the involvement of primary metabolism in the overproduction of native and atypical metabolites, including nutraceuticals—compounds of great interest due to their health-promoting or disease-preventing properties—thus overcoming limitations of traditional low-yield extractions of natural materials or of complex chemical syntheses.
Omega-3 fatty acids, all cis-5,8,11,14,17-eicosapentaenoic (C20:5, EPA), and all-cis-4,7,10,13,16,19-docosahexaenoic (C22:6, DHA) acids provide great benefits in improving human health in adults and developing infants. Currently, the main source of EPA and DHA is fish oil, and microalgae are also used as a secondary source of DHA. However, because of the high demand for EPA and DHA, many synthetic methods have been developed for producing these and other ω-3 fatty acids, including metabolically engineered strains of oleaginous yeasts, especially Yarrowia lipolytica. This review describes current metabolic engineering strategies used in the manufacture of some nutraceuticals, especially high levels of polyunsaturated fatty acids (PUFAs).
Permanent Link: https://hdl.handle.net/11104/0336085