Tuning of surface charge of functionalized poly(carboxybetaine) brushes can significantly improve label-free biosensing in complex media

0564268 - FZÚ 2023 RIV DE eng J - Journal Article
Víšová, Ivana - Houska, Milan - Spasovová, Monika - Forinová, Michala - Pilipenco, Alina - Mezuláníková, K. - Tomandlová, Markéta - Mrkvová, Kateřina - Vrabcová, Markéta - Dejneka, Alexandr - Dostálek, Jakub - Vaisocherová-Lísalová, Hana
Tuning of surface charge of functionalized poly(carboxybetaine) brushes can significantly improve label-free biosensing in complex media.
Advanced Materials Interfaces. Roč. 9, č. 33 (2022), č. článku 2201210. ISSN 2196-7350. E-ISSN 2196-7350
R&D Projects: GA ČR(CZ) GA21-19779S; GA MŠMT(CZ) EF18_053/0016627
Grant - others:OP VVV - Mobility FZU 2(XE) CZ.02.2.69/0.0/0.0/18_053/0016627; AV ČR(CZ) LQ100101902
Program: Prémie Lumina quaeruntur
Institutional support: RVO:68378271
Keywords : antifouling zwitterionic polymer brushes * biosensors * EDC/NHS chemistry * N-hydroxysuccinimide (NHS) active ester deactivation * poly(carboxybetaine) functionalization
OECD category: Biophysics
Impact factor: 5.4, year: 2022
Method of publishing: Limited access
https://doi.org/10.1002/admi.202201210

Poly(carboxybetaine) brushes are excellent antifouling platforms allowing facile functionalization with biorecognition elements via carbodiimide/N-hydroxysuccin- imide (EDC/NHS) chemistry. However, residual active NHS esters and the loss of zwitterionic balance after the conjugation may impair initially excellent antifouling properties. This problem has so far been addressed either by using spontaneous hydrolysis or deactivation of residual NHS esters by the reaction with a small amino compound bearing hydroxyl or carboxyl groups. In contrast to this approach, and instead of using a single deactivator, here the use of tailored mixtures of deacti- vating agents containing carboxyl groups and sulfo or sulfate groups with perma- nent negative charge that allow to tune surface charge balance is investigated.

Permanent Link: https://hdl.handle.net/11104/0335971