Preparative 3D printed device for the short DNA fragment separation

0564101 - ÚIACH 2023 CZ eng A - Abstract
Hrušková, Helena - Řemínek, Roman - Foret, František
Preparative 3D printed device for the short DNA fragment separation.
Proceedings of APCE-CECE-ITP-IUPAC 2022. Brno: Ústav analytické chemie AV ČR, v. v. i., 2022 - (Foret, F.; Lavická, J.; Chung, D.). s. 177-177. ISBN 978-80-904959-9-9.
[APCE-CECE-ITP-IUPAC 2022. 06.11.2022-10.11.2022, Angkor Wat]
R&D Projects: GA TA ČR(CZ) FW02020209
Institutional support: RVO:68081715
Keywords : 3D-printed device * blood plasma samples * DNA fragments
OECD category: Analytical chemistry

We developed a new 3D-printed device for preparative precleaning blood plasma samples from larger DNA fragments. Such a sample adjustment should increase the quality of data obtained from subsequent PCR, where only particular short DNA fragments are considered to be biomarkers of the cancer disease. In our study, the device was designed, fabricated by 3D-printing from the polymeric material HD PLA, and tested. A method based on electromigration separation techniques for the preparative separation of short DNA fragments from larger fragments has been developed and partially validated.

Permanent Link: https://hdl.handle.net/11104/0335817