The relation between protein adsorption and hemocompatibility of antifouling polymer brushes

0564084 - ÚMCH 2023 RIV DE eng J - Journal Article
Riedelová, Zuzana - de los Santos Pereira, Andres - Svoboda, Jan - Pop-Georgievski, Ognen - Májek, P. - Pečánková, K. - Dyčka, F. - Rodriguez-Emmenegger, C. - Riedel, Tomáš
The relation between protein adsorption and hemocompatibility of antifouling polymer brushes.
Macromolecular Bioscience. Roč. 22, č. 11 (2022), č. článku 2200247. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA ČR(CZ) GA20-10845S; GA ČR(CZ) GF21-16729K
Grant - others:AV ČR(CZ) MSM200502001
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:61389013
Keywords : antifouling surfaces * hemocompatibility * MS identification
OECD category: Biophysics
Impact factor: 4.6, year: 2022
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/10.1002/mabi.202200247

Whenever an artificial surface comes into contact with blood, proteins are rapidly adsorbed onto its surface. This phenomenon, termed fouling, is then followed by a series of undesired reactions involving activation of complement or the coagulation cascade and adhesion of leukocytes and platelets leading to thrombus formation. Thus, considerable efforts are directed towards the preparation of fouling-resistant surfaces with the best possible hemocompatibility. Herein, a comprehensive hemocompatibility study after heparinized blood contact with seven polymer brushes prepared by surface-initiated atom transfer radical polymerization is reported. The resistance to fouling is quantified and thrombus formation and deposition of blood cellular components on the coatings are analyzed. Moreover, identification of the remaining adsorbed proteins is performed via mass spectroscopy to elucidate their influence on the surface hemocompatibility. Compared with an unmodified glass surface, the grafting of polymer brushes minimizes the adhesion of platelets and leukocytes and prevents the thrombus formation. The fouling from undiluted blood plasma is reduced by up to 99%. Most of the identified proteins are connected with the initial events of foreign body reaction towards biomaterial (coagulation cascade proteins, complement component, and inflammatory proteins). In addition, several proteins that are not previously linked with blood-biomaterial interaction are presented and discussed.

Permanent Link: https://hdl.handle.net/11104/0336175