MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis

0563830 - MBÚ 2023 RIV US eng J - Journal Article
de Luxan-Hernandez, C. - Lohmann, J. - Tranque, E. - Chumová, Jana - Binarová, Pavla - Salinas, J. - Weingartner, M.
MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis.
Life Science Alliance. Roč. 6, č. 1 (2022), č. článku e202201507. E-ISSN 2575-1077
Grant - others:AV ČR(CZ) DAAD-19-04; AV ČR(CZ) DAAD-21-07
Program: Bilaterální spolupráce; Bilaterální spolupráce
Institutional support: RVO:61388971
Keywords : dna-damage response * sr-related proteins * u4/u6.u5 tri-snrnp * lsm proteinsfunctional-analysisrnaidentificationspliceosomeatrphosphoproteomics * lsm proteins * functional-analysis * rna * identificationspliceosomeatrphosphoproteomics * identification * spliceosome * atr * phosphoproteomics
OECD category: Microbiology
Impact factor: 4.4, year: 2022
Method of publishing: Open access
https://www.life-science-alliance.org/content/6/1/e202201507

The coordination of cell division with stress response is essential for maintaining genome stability in plant meristems. Proteins involved in pre-mRNA splicing are important for these processes in animal and human cells. Based on its homology to the splicing factor SART1, which is implicated in the control of cell division and genome stability in human cells, we analyzed if MDF has similar functions in plants. We found that MDF associates with U4/U6.U5 tri-snRNP proteins and is essential for correct splicing of 2,037 transcripts. Loss of MDF function leads to cell division defects and cell death in meristems and was associated with up-regulation of stress-induced genes and down-regulation of mitotic regulators. In addition, the mdf-1 mutant is hypersensitive to DNA damage treatment supporting its role in coordinating stress response with cell division. Our analysis of a dephosphomutant of MDF suggested how its protein activity might be controlled. Our work uncovers the conserved function of a plant splicing factor and provides novel insight into the interplay of pre-mRNA processing and genome stability in plants.
Permanent Link: https://hdl.handle.net/11104/0335624