Gas-Plasma-Activated Water Impact on Photo-Dependent Dormancy Mechanisms in Nicotiana tabacum Seeds

0563336 - ÚEB 2023 RIV CH eng J - Journal Article
Grainge, G. - Nakabayashi, K. - Iza, F. - Leubner-Metzger, Gerhard - Steinbrecher, T.
Gas-Plasma-Activated Water Impact on Photo-Dependent Dormancy Mechanisms in Nicotiana tabacum Seeds.
International Journal of Molecular Sciences. Roč. 23, č. 12 (2022), č. článku 6709. E-ISSN 1422-0067
Institutional support: RVO:61389030
Keywords : gas-plasma-activated water * germination * gibberellin oxidase * photo-dependent dormancy * seed dormancy * tobacco
OECD category: Plant sciences, botany
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://doi.org/10.3390/ijms23126709

Seeds sense temperature, nutrient levels and light conditions to inform decision making on the timing of germination. Limited light availability for photoblastic species results in irregular germination timing and losses of population germination percentage. Seed industries are therefore looking for interventions to mitigate this risk. A growing area of research is water treated with gas plasma (GPAW), in which the formed solution is a complex consisting of reactive oxygen and nitrogen species. Gas plasma technology is widely used for sterilisation and is an emerging technology in the food processing industry. The use of the GPAW on seeds has previously led to an increase in germination performance, often attributed to bolstered antioxidant defence mechanisms. However, there is a limited understanding of how the solution may influence the mechanisms that govern seed dormancy and whether photoreceptor-driven germination mechanisms are affected. In our work, we studied how GPAW can influence the mechanisms that govern photo-dependent dormancy, isolating the effects at low fluence response (LFR) and very low fluence response (VLFR). The two defined light intensity thresholds affect germination through different phytochrome pho-toreceptors, PHYB and PHYA, respectively, we found that GPAW showed a significant increase in population germination percentage under VLFR and further described how each treatment affects key physiological regulators.
Permanent Link: https://hdl.handle.net/11104/0335323