Programmable Millifluidic Platform Integrating Automatic Electromembrane Extraction Cleanup and In-Line Electrochemical Detection: A Proof of Concept

0563043 - ÚIACH 2023 RIV US eng J - Journal Article
Sahragard, A. - Dvořák, Miloš - Carrasco-Correa, E. J. - Varanasupakul, P. - Kubáň, Pavel - Miró, M.
Programmable Millifluidic Platform Integrating Automatic Electromembrane Extraction Cleanup and In-Line Electrochemical Detection: A Proof of Concept.
ACS Sensors. Roč. 7, č. 10 (2022), s. 3161-3168. ISSN 2379-3694. E-ISSN 2379-3694
Institutional support: RVO:68081715
Keywords : electrochemical sensing * diclofenac * sequential injection analysis
OECD category: Analytical chemistry
Impact factor: 8.9, year: 2022
Method of publishing: Open access

A fully automatic millifluidic sensing platform coupling in-line nonsupported microelectromembrane extraction (mu-EME) with electrochemical detection (ECD) is herein proposed for the first time. Exploiting the features of the second generation of flow analysis, termed sequential injection (SI), the smart integration of SI and mu-EME-ECD enables (i) the repeatable formation of microvolumes of phases for the extraction step in a membrane-less (nonsupported) arrangement, (ii) diverting the acceptor plug to the ECD sensing device, (iii) in-line pH adjustment before the detection step, and (iv) washing of the platform for efficient removal of remnants of wetting film solvent, all entirely unsupervised. The real-life applicability of the miniaturized sensing system is studied for in-line sample cleanup and ECD of diclofenac as a model analyte after mu-EME of urine as a complex biological sample. A comprehensive study of the merits and the limitations of mu-EME solvents on ECD is presented. Under the optimal experimental conditions using 14 mu L of unprocessed urine as the donor, 14 mu L of 1-nonanol as the organic phase, and 14 mu L of 25 mM NaOH as the acceptor in a 2.4 mm ID PTFE tubing, an extraction voltage of 250 V, and an extraction time of 10 min, an absolute (mass) extraction recovery of 48% of diclofenac in urine is obtained. The proposed flow through system is proven to efficiently remove the interfering effect of predominantly occurring organic species in human urine on ECD with RSD% less than 8.6%.
Permanent Link: https://hdl.handle.net/11104/0335129