Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods

0563014 - ÚŽFG 2023 RIV GB eng J - Journal Article
Vaníčková, L. - Do, T. - Vejvodová, M. - Horák, Vratislav - Hubálek, Martin - Emri, G. - Zemánková, K. - Pavelicová, K. - Křížková, S. - Faltusová, V. - Pompeiano, A. - Vaculovičová, M. - Zítka, O. - Vaculovič, T. - Adam, V.
Mapping of MeLiM melanoma combining ICP-MS and MALDI-MSI methods.
International Journal of Biological Macromolecules. Roč. 203, Apr 1 (2022), s. 583-592. ISSN 0141-8130. E-ISSN 1879-0003
R&D Projects: GA MŠMT(CZ) EF16_019/0000785
Institutional support: RVO:67985904 ; RVO:61388963
Keywords : melanoma * mass spectrometry * MeLiM
OECD category: Oncology; Analytical chemistry (UOCHB-X)
Impact factor: 8.2, year: 2022
Method of publishing: Limited access
https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=91749918693

Here we developed a powerful tool for comprehensive data collection and mapping of molecular and elemental signatures in the Melanoma-bearing Libechov Minipig (MeLiM) model. The combination of different mass spectrometric methods allowed for detail investigation of specific melanoma markers and elements and their spatial distribution in tissue sections. MALDI-MSI combined with HPLC-MS/MS analyses resulted in identification of seven specific proteins, S100A12, CD163, MMP-2, galectin-1, tenascin, resistin and PCNA that were presented in the melanoma signatures. Furthermore, the ICP-MS method allowed for spatial detection of zinc, calcium, copper, and iron elements linked with the allocation of the specific binding proteins.
Permanent Link: https://hdl.handle.net/11104/0335110