Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle

0562690 - BÚ 2023 RIV NL eng J - Journal Article
Čertnerová, D. - Čertner, Martin - Škaloud, P.
Alternating nuclear DNA content in chrysophytes provides evidence of their isomorphic haploid-diploid life cycle.
Algal Research-Biomass Biofuels and Bioproducts. Roč. 64, MAY (2022), č. článku 102707. ISSN 2211-9264. E-ISSN 2211-9264
Institutional support: RVO:67985939
Keywords : isomorphic haploid-diploid life cycle * chrysophytes * flow cytometry
OECD category: Plant sciences, botany
Impact factor: 5.1, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.algal.2022.102707

In many unicellular eukaryotes (protists), the life cycles are still largely unexplored, although this knowledge is key to understanding their biology. We used flow cytometry to detect the often-inconspicuous transitions among life cycle stages in three genera of chrysophyte algae (Chrysosphaerella, Ochromonas, and Synura). We revealed an alternation of two ploidy levels over time in strains maintained in long-term cultivation, both life cycle stages were capable of mitotic growth and shared similar phenotypes. Therefore, chrysophytes have a haploid-diploid life cycle with isomorphic life cycle stages. As far as we know, this is the first report of such life cycle strategy in unicellular algae. Life cycle stages and life stage transitions seem to be synchronized among all cells coexisting within a culture, possibly due to chemical signals. Additionally, ploidy level variation resulting from whole genome doubling was identified in two species of Synura.
Permanent Link: https://hdl.handle.net/11104/0336823