iP & OEIP - Cytokinin Micro Application Modulates Root Development with High Spatial Resolution

0562425 - ÚEB 2023 RIV US eng J - Journal Article
Pařízková, Barbora - Antoniadi, I. - Poxson, D. J. - Karady, Michal - Simon, D. T. - Zatloukal, M. - Strnad, Miroslav - Doležal, Karel - Novák, Ondřej - Ljung, K.
iP & OEIP - Cytokinin Micro Application Modulates Root Development with High Spatial Resolution.
Advanced Materials Technologies. Roč. 7, č. 10 (2022), č. článku 2101664. ISSN 2365-709X. E-ISSN 2365-709X
R&D Projects: GA MŠMT(CZ) EF16_019/0000827; GA MŠMT(CZ) EF16_019/0000738; GA ČR(CZ) GA19-00973S
Institutional support: RVO:61389030
Keywords : arabidopsis * cytokinin * hormone delivery * lateral root * organic bioelectronics * root development * spatial resolution
OECD category: Biochemistry and molecular biology
Impact factor: 6.8, year: 2022
Method of publishing: Open access
https://doi.org/10.1002/admt.202101664

State-of-the-art technology based on organic electronics can be used as a flow-free delivery method for organic substances with high spatial resolution. Such highly targeted drug micro applications can be used in plant research for the regulation of physiological processes on tissue and cellular levels. Here, for the first time, an organic electronic ion pump (OEIP) is reported that can transport an isoprenoid-type cytokinin, N6-isopentenyladenine (iP), to intact plants. Cytokinins (CKs) are plant hormones involved in many essential physiological processes, including primary root (PR) and lateral root (LR) development. Using the Arabidopsis thaliana root as a model system, efficient iP delivery is demonstrated with a biological output – cytokinin-related PR and LR growth inhibition. The spatial resolution of iP delivery, defined for the first time for an organic compound, is shown to be less than 1 mm, exclusively affecting the OEIP-targeted LR. Results from the application of the high-resolution OIEP treatment method confirm previously published findings showing that the influence of CKs may vary at different stages of LR development. Thus, OEIP-based technologies offer a novel, electronically controlled method for phytohormone delivery that could contribute to unraveling cytokinin functions during different developmental processes with high specificity.
Permanent Link: https://hdl.handle.net/11104/0334741