Fine mapping of powdery mildew and stripe rust resistance genes Pm5V/Yr5V transferred from Dasypyrum villosum into wheat without yield penalty

0562372 - ÚEB 2023 RIV DE eng J - Journal Article
Zhang, R. - Lu, C. - Meng, X. - Fan, Y. - Du, J. - Liu, R. - Feng, Y. - Xing, L. - Cápal, Petr - Holušová, Kateřina - Doležel, Jaroslav - Wang, Y. - Mu, H. - Sun, B. - Hou, F. - Yao, R. - Xiong, C. - Wang, Y. - Chen, P. - Cao, A.
Fine mapping of powdery mildew and stripe rust resistance genes Pm5V/Yr5V transferred from Dasypyrum villosum into wheat without yield penalty.
Theoretical and Applied Genetics. Roč. 135, č. 10 (2022), s. 3629-3642. ISSN 0040-5752. E-ISSN 1432-2242
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : Basidiomycetes * disease resistance * Mapping
OECD category: Biochemistry and molecular biology
Impact factor: 5.4, year: 2022
Method of publishing: Open access
https://doi.org/10.1007/s00122-022-04206-9

The powdery mildew and stripe rust seriously threaten wheat production worldwide. Dasypyrum villosum (2n = 2x = 14, VV), a relative of wheat, is a valuable resource of resistance genes for wheat improvement. Here, we describe a platform for rapid introgression of the resistance genes from D. villosum into the wheat D genome. A complete set of new wheat-D. villosum V (D) disomic substitution lines and 11 D/V Robertsonian translocation lines are developed and characterized by molecular cytogenetic method. A new T5DL·5V#5S line NAU1908 shows resistance to both powdery mildew and stripe rust, and the resistances associated with 5VS are confirmed to be conferred by seedling resistance gene Pm5V and adult-plant resistance gene Yr5V, respectively. We flow-sort chromosome arm 5VS and sequence it using the Illumina NovaSeq 6000 system that allows us to generate 5VS-specific markers for genetic mapping of Pm5V/Yr5V. Fine mapping shows that Pm5V and Yr5V are closely linked and the location is narrowed to an approximately 0.9 Mb region referencing the sequence of Chinese Spring 5DS. In this region, a NLR gene in scaffold 24,874 of 5VS orthologous to TraesCS5D02G044300 is the most likely candidate gene for Pm5V. Soft- and hard-grained T5DL·5V#5S introgressions confer resistance to both powdery mildew and stripe rust in diverse wheat genetic backgrounds without yield penalty. Meanwhile, significant decrease in plant height and increase in yield were observed in NIL-5DL·5V#5S compared with that in NIL-5DL·5DS. These results indicate that Pm5V/Yr5V lines might have the potential value to facilitate wheat breeding for disease resistance.
Permanent Link: https://hdl.handle.net/11104/0334725