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The humidity level matters during the desiccation of Norway spruce somatic embryos

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    0562063 - ÚEB 2023 RIV CH eng J - Journal Article
    Fischerová, Lucie - Gemperlová, Lenka - Cvikrová, Milena - Matušíková, I. - Moravčíková, J. - Gerši, Z. - Malbeck, Jiří - Kuderna, Jan - Pavlíčková, Jana - Motyka, Václav - Eliášová, Kateřina - Vondráková, Zuzana
    The humidity level matters during the desiccation of Norway spruce somatic embryos.
    Frontiers in Plant Science. Roč. 13, JUL 29 (2022), č. článku 968982. ISSN 1664-462X. E-ISSN 1664-462X
    R&D Projects: GA MŠMT(CZ) LTC17036
    Grant - others:Czech and Slovak Academy of Sciences(CZ) SAV-AVČR 15-06
    Institutional support: RVO:61389030
    Keywords : 3-glucanases * chitinases * desiccation * germination * Picea abies (L.) Karst * polyamines * somatic embryogenesis * beta-1,3-glucanases
    OECD category: Plant sciences, botany
    Impact factor: 5.6, year: 2022
    Method of publishing: Open access
    https://doi.org/10.3389/fpls.2022.968982

    In Norway spruce, as in many other conifers, the germination capacity of somatic embryos is strongly influenced by the desiccation phase inserted after maturation. The intensity of drying during desiccation eminently affected the formation of emblings (i.e., seedlings developed from somatic embryos). Compared to non-desiccated embryos, the germination capacity of embryos desiccated at 100% relative humidity was about three times higher, but the reduction of relative humidity to 95 and 90% had a negative effect on the subsequent embryo development. The water loss observed in these embryos did not lead to an increase in lipid peroxidation, as shown by malondialdehyde levels. Another metabolic pathway in plants that mediates a response to abiotic stresses is directed toward the biosynthesis of polyamines (PAs). The activities of PA biosynthetic enzymes increased steadily in embryos during desiccation at 100% relative humidity, whereas they decreased at lower humidity. The total content of free PAs in the embryos gradually decreased throughout desiccation. The increase in free putrescine (Put) and perchloric acid-insoluble Put conjugates was observed in embryos desiccated at lower humidity. These changes were accompanied to some extent by the transcription of the genes for the PA biosynthesis enzymes. Desiccation at 100% relative humidity increased the activity of the cell wall-modifying enzymes β-1,3-glucanases and chitinases, the activities of these enzymes were also significantly suppressed at reduced humidity. The same pattern was observed in the transcription of some β-1,3-glucanase and chitinase genes. Desiccation treatments triggered metabolic processes that responded to water availability, suggesting an active response of the embryo to the reduction in humidity. A positive effect was demonstrated only for desiccation at high relative humidity. Some of the physiological characteristics described can be used as markers of inappropriate relative humidity during somatic embryo desiccation.
    Permanent Link: https://hdl.handle.net/11104/0334479

     
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