Impact of electrolyte solution on electrochemical oxidation treatment of Escherichia coli K-12 by boron-doped diamond electrodes

0561908 - FZÚ 2023 RIV GB eng J - Journal Article
Budil, Jakub - Szabó, Ondrej - Lišková, P. - Štenclová, Pavla - Izsák, Tibor - Potocký, Štěpán - Kromka, Alexander
Impact of electrolyte solution on electrochemical oxidation treatment of Escherichia coli K-12 by boron-doped diamond electrodes.
Letters in Applied Microbiology. Roč. 74, č. 6 (2022), s. 924-931. ISSN 0266-8254. E-ISSN 1472-765X
R&D Projects: GA MŠMT(CZ) EF16_019/0000760; GA MŠMT LM2018110
Grant - others:OP VVV - SOLID21(XE) CZ.02.1.01/0.0/0.0/16_019/0000760
Institutional support: RVO:68378271
Keywords : boron-doped diamond * chloride-based electrolyte * chloride-free electrolyte * Escherichia coli * water disinfection
OECD category: Microbiology
Impact factor: 2.4, year: 2022
Method of publishing: Open access

We studied the disinfection efficacy of boron-doped electrodes on Escherichia coli-contaminated water-based solutions in three different electrolytes, physiological solution (NaCl), phosphate buffer (PB), and phosphate buffer saline (PBS). The effect of the electrochemical oxidation treatment on the bacteria viability was studied by drop and spread plate cultivation methods, and supported by optical density measurements. We have found that bacterial suspensions in NaCl and PBS underwent a total inactivation of all viable bacteria within 10 min of the electrochemical treatment. By contrast, experiments performed in the PB showed a relatively minor decrease of viability by two orders of magnitude after 2 h of the treatment, which is almost comparable with the untreated control. The enhanced bacterial inactivation was assigned to reactive chlorine species, capable of penetrating the bacterial cytoplasmic membrane and killing bacteria from within.
Permanent Link: https://hdl.handle.net/11104/0334331