Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants

0561543 - ÚEB 2023 RIV GB eng J - Journal Article
Dünser, K. - Schöller, M. - Rößling, A. K. - Löfke, C. - Xiao, N. - Pařízková, Barbora - Melnik, S. - Rodriguez-Franco, M. - Stöger, E. - Novák, Ondřej - Kleine-Vehn, J.
Endocytic trafficking promotes vacuolar enlargements for fast cell expansion rates in plants.
eLife. Roč. 11, JUN 10 (2022), č. článku e75945. ISSN 2050-084X. E-ISSN 2050-084X
R&D Projects: GA MŠMT(CZ) EF16_019/0000827
Institutional support: RVO:61389030
Keywords : small molecules * vacuole * plasma membrane
OECD category: Biochemistry and molecular biology
Impact factor: 7.7, year: 2022
Method of publishing: Open access
https://doi.org/10.7554/eLife.75945

The vacuole has a space-filling function, allowing a particularly rapid plant cell expansion with very little increase in cytosolic content (Löfke et al., 2015, Scheuring et al., 2016, Dünser et al., 2019). Despite its importance for cell size determination in plants, very little is known about the mechanisms that define vacuolar size. Here, we show that the cellular and vacuolar size expansions are coordinated. By developing a pharmacological tool, we enabled the investigation of membrane delivery to the vacuole during cellular expansion. Our data reveal that endocytic membrane sorting from the plasma membrane to the vacuole is enhanced in the course of rapid root cell expansion. While this ‘compromise’ mechanism may theoretically at first decelerate cell surface enlargements, it fuels vacuolar expansion and, thereby, ensures the coordinated augmentation of vacuolar occupancy in dynamically expanding plant cells.
Permanent Link: https://hdl.handle.net/11104/0334129