In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A

0561138 - ÚŽFG 2023 RIV NL eng J - Journal Article
Strnádel, J. - Dumortier, H. - Hajduchová, D. - Záhumenská, R. - Nosál, V. - Smolár, M. - Marcínek, J. - Kalman, M. - Mersáková, S. - Braný, D. - Juhás, Štefan - Juhásová, Jana - Studenovská, Hana - Mitrušková, B. - Suroviaková, S. - Nováková, S. - Skovierová, H. - Kurča, E. - Pecová, R. - Plank, L. - Halašová, E.
In vitro modeling of amyotrophic lateral sclerosis with induced pluripotent stem cell technology-derived cell line ORIONi002-A.
Stem Cell Research. Roč. 63, AUG 2022 (2022), č. článku 102870. ISSN 1873-5061. E-ISSN 1876-7753
Institutional support: RVO:67985904 ; RVO:61389013
Keywords : amyotrophic lateral sclerosis * stem cell
OECD category: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction); Neurosciences (including psychophysiology (UMCH-V)
Impact factor: 1.2, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S1873506122002197?via%3Dihub

We present here a new iPS cell line for modeling sporadic form of ALS. Cell line was generated by reprogramming skin fibroblasts isolated with explant culture technology from skin biopsy, donated by ALS patient. For reprogramming, polycistronic self-replicating RNA vector was used and derived iPS cells were charac-terized by immunocytochemistry and FACS (pluripotent factors expression), karyotyping, STR fingerprinting analysis and in vitro differentiation assay. New cell line showed normal (46, XY) karyotype and differentiated in vitro into cells from three germ layers. STR analysis proved the origin and originality of the cell line.
Permanent Link: https://hdl.handle.net/11104/0333822