Green extraction processing of lutein from Chlorella saccharophila in water-based ionic liquids as a sustainable innovation in algal biorefinerie

0561084 - MBÚ 2023 RIV NL eng J - Journal Article
Paliwa, Chetan - Rehmanji, M. - Shaikha, K. M. - Zafar, S. U. - Jutur, P. P.
Green extraction processing of lutein from Chlorella saccharophila in water-based ionic liquids as a sustainable innovation in algal biorefinerie.
Algal Research-Biomass Biofuels and Bioproducts. Roč. 66, JUL 2022 (2022), č. článku 102809. ISSN 2211-9264. E-ISSN 2211-9264
Institutional support: RVO:61388971
Keywords : Algae * Lutein * Carotenoids * Extraction * Ionic liquids * Thermal stability
OECD category: Microbiology
Impact factor: 5.1, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S2211926422001801?via%3Dihub

A billion tonnes of waste are generated every year in the food industry. Green extraction of natural antioxidants and therapeutics like carotenoids from microalgae using ionic liquids may help in improving the process efficiency and reducing the dependency on petroleum-based chemicals in the food industry. The extraction of ca-rotenoids is solvent specific, and extraction efficiency varies greatly. The effect of 4 different ILs on carotenoid profiles of C. saccharophila was assessed and compared to a conventional solvent, methanol, to check their extractability. We found 40 % (w/v) Tetrabutyl phosphonium hydroxide as the best IL to extract the carotenoids. Tetrabutyl phosphonium hydroxide selectively extracts 2.26 mg g(-1) dry cell weight (DCW) of lutein from wet biomass of C. saccharophila, whereas methanol extracts 0.10 mg g(-1) DCW of lutein at room temperature-based extraction. Also, the combinatorial effect of temperature, extraction time, solvent: biomass ratio and ionic liquid concentration was investigated using a statistical modelling technique, Response Surface Modelling (RSM). It was found that lutein extraction is further improved by 10 % to 2.49 mg g(-1) under the optimal extraction conditions [40 % (w/v) Ionic liquid concentration, 5 min extraction time, 0.5 (mL mg(-1)) Solvent: Biomass Ratio and 25 degrees C temperature]. Lutein remains 3 times more stable in IL than in conventional solvent methanol at 60 and 90 degrees C. Hence, Tetrabutyl phosphonium hydroxide can prospectively substitute conventional organic solvents in the downstream processing of bioactive compounds from microalgae
Permanent Link: https://hdl.handle.net/11104/0333887