Biological autoluminescence as a non-invasive monitoring tool for pulsed electric field effects on yeast cells

0560868 - FZÚ 2023 RIV US eng C - Conference Paper (international conference)
Bereta, M. - Teplan, M. - Chafai, Djamel Eddine - Cifra, Michal
Biological autoluminescence as a non-invasive monitoring tool for pulsed electric field effects on yeast cells.
33rd General Assembly and Scientific Symposium (GASS) of the International-Union-of-Radio-Science (URSI) 2020. New York: IEEE, 2020, č. článku 9232161. ISBN 978-9-4639-6800-3.
[General Assembly and Scientific Symposium (GASS) of the International-Union-of-Radio-Science (URSI) /33./. Řím (IT), 29.08.2020-05.08.2020]
R&D Projects: GA ČR GA18-23597S
Grant - others:AV ČR(CZ) SAV-18-11
Program: Bilaterální spolupráce
Institutional support: RVO:68378271 ; RVO:67985882
Keywords : biology * electric fields * monitoring * biomedical measurement * photonics * tools * pulse measure
OECD category: Medical laboratory technology (including laboratory samples analysis; Electrical and electronic engineering (URE-Y)
https://ieeexplore.ieee.org/document/9232161/authors#authors

The application of pulsed electric field (PEF) is nowadays becoming a very promising tool for application in medicine or food industry. However, the mechanisms of PEF interaction with living matter are still not fully elucidated. The aim of the presented work is to show the potential application of biological autoluminescence (BAL) for monitoring of pulsed electric field (PEF) biological effects on yeasts. The yeast cell culture (Saccharomyces cerevisiae) is exposed to the pulsed electric field (pulse duration: 0.1-5 ms, pulse number: 1-10, electric field strength: 3 kV.cm(-1)) and the BAL is monitored during exposure. The results indicate detectable differences in BAL dynamics when PEF is applied. Due to its non-invasive and label-free application, the BAL could be used as a beneficial tool for monitoring of PEF biological effects.
Permanent Link: https://hdl.handle.net/11104/0333650