Rose Bengal-modified upconverting nanoparticles: synthesis, characterization, and biological evaluation

0560844 - ÚMCH 2023 RIV CH eng J - Journal Article
Nahorniak, Mykhailo - Pop-Georgievski, Ognen - Velychkivska, Nadiia - Filipová, Marcela - Rydvalová, Eliška - Gunár, Kristýna - Matouš, P. - Kostiv, Uliana - Horák, Daniel
Rose Bengal-modified upconverting nanoparticles: synthesis, characterization, and biological evaluation.
Life. Roč. 12, č. 9 (2022), č. článku 1383. E-ISSN 2075-1729
R&D Projects: GA ČR(CZ) GA21-04420S; GA ČR(CZ) GA20-07313S
Institutional support: RVO:61389013
Keywords : upconverting * nanoparticles * Rose Bengal
OECD category: Polymer science
Impact factor: 3.2, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2075-1729/12/9/1383

High-quality upconverting NaYF4:Yb3+,Er3+ nanoparticles (UCNPs, 26 nm in diameter) based on lanthanides were synthesized by a high-temperature coprecipitation method. The particles were modified by bisphosphonate-terminated poly(ethylene glycol) (PEG) and Rose Bengal (RB) photosensitizer. The particles were thoroughly characterized using transmission electron microscopy, dynamic light scattering, thermogravimetric analysis, FTIR, and X-ray photoelectron and upconversion luminescence spectroscopy in terms of morphology, hydrodynamic size, composition, and energy transfer to the photosensitizer. Moreover, the singlet oxygen generation from RB-containing UCNPs was investigated using 9,10-diphenylanthracene probe under 980 nm excitation. The cytotoxicity of UCNPs before and after conjugation with RB was evaluated on highly sensitive rat mesenchymal stem cells (rMSCs) and significant differences were found. Correspondingly, consi-derable variations in viability were revealed between the irradiated and non-irradiated rat glioma cell line (C6) exposed to RB-conjugated UCNPs. While the viability of rMSCs was not affected by the presence of UCNPs themselves, the cancer C6 cells were killed after the irradiation at 980 nm due to the reactive oxygen species (ROS) production, thus suggesting the potential of RB-conjugated PEG-modified UCNPs for applications in photodynamic therapy of cancer.
Permanent Link: https://hdl.handle.net/11104/0334452