Extension of the Internal Standard Method for Determination of Thermodynamic Acidity Constants of Compounds Sparingly Soluble in Water by Capillary Zone Electrophoresis

0560706 - BÚ 2023 RIV US eng J - Journal Article
Nytrová, L. - Odehnalová, Klára - Pazourek, J.
Extension of the Internal Standard Method for Determination of Thermodynamic Acidity Constants of Compounds Sparingly Soluble in Water by Capillary Zone Electrophoresis.
ACS Omega. Roč. 7, č. 1 (2022), s. 1477-1482. ISSN 2470-1343. E-ISSN 2470-1343
Institutional support: RVO:67985939
Keywords : high-throughput method * dissociation constants * determination mobilities values * drugs
OECD category: Analytical chemistry
Impact factor: 4.1, year: 2022
Method of publishing: Open access
https://doi.org/10.1021/acsomega.1c06224

The paper extends applicability of the internal standard method published in 2009 (Fuguet E. et al., J. Chromatogr. A 2009, 1216(17), 3646). Although the original capillary zone electrophoresis method was suggested to determine thermodynamic acidity constants of compounds sparingly soluble in aqueous solutions by carrying out only runs at two different pH values (i.e., without the need to perform many experiments over the appropriate pH range including the form of a low-ionized analyte), we proved that the approach also virtually overcomes any interactions of the analyte in mixed solvents, so that the experiments can be carried out in a methanol-water buffer where the solubility is much better. Applicability of the extended method is illustrated on six selected beta-blockers.
Permanent Link: https://hdl.handle.net/11104/0333575