Revisiting bioactivity of calcium titanate and titanium dioxide: Hydrolysis and complexation effects in osteogenic medium

0560435 - GLÚ 2023 RIV CH eng J - Journal Article
Křenek, T. - Stich, T. - Docheva, D. - Mikysek, Petr - Koštejn, Martin - Jandová, Věra - Fajgar, Radek - Pola, M. - Kovářík, T. - Moskal, D. - Forejtová, M. - Pola, Josef
Revisiting bioactivity of calcium titanate and titanium dioxide: Hydrolysis and complexation effects in osteogenic medium.
Surface and Coatings Technology. Roč. 447, October (2022), č. článku 128820. ISSN 0257-8972
Institutional support: RVO:67985831 ; RVO:67985858
Keywords : Calcium titanate coat * Hydrolysis * Micro/nanostructured oxidic titanium * hMSCs * Osteogenic differentiation * DMEM-TiO₂ interactions * Self-organized precipitation of DMEM
OECD category: Mineralogy; Inorganic and nuclear chemistry (UCHP-M)
Impact factor: 5.4, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S0257897222007411

This study characterized laser-deposited CaTiO₃ coats on several substrates by electron microscopy, X-ray diffraction, and Raman spectra. The hydrolytic stability of such coats exposed to ambient air and immersed into culture and osteogenic media has been respectively assessed by X-ray photoelectron spectra and energy dispersive X-ray analysis. It is shown these coats on titanium and micro/nanostructured oxidic titanium are not stable in air and culture or osteogenic media where they undergo hydrolysis and Ca leakage. The role of this hydrolysis is assessed and discussed in terms of not yet acknowledged complexation of the developing TiO₂ surface with constituents of Dulbecco's Modified Eagle's medium. The effect of the deposited and additionally heated CT coatings on human mesenchymal stem cells is assessed regarding survival, proliferation and differentiation towards the osteogenic lineage in osteogenic medium. It is also stressed that the identified CaTiO₃ hydrolysis in air and aquatic media seriously impacts the interpretation of bioactivity and physicochemical, catalytic and photocatalytic properties of CaTiO₃ surfaces, which has not been yet admitted and led to erroneous conclusions in many previous studies.
Permanent Link: https://hdl.handle.net/11104/0334251