Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study

0560417 - ÚEM 2023 RIV CH eng J - Journal Article
Canciani, E. - Gagliano, N. - Paino, F. - Amler, Evžen - Divín, Radek - Denti, L. - Henin, D. - Fiorati, A. - Dellavia, C.
Polyblend Nanofibers to Regenerate Gingival Tissue: A Preliminary In Vitro Study.
Frontiers in Materials. Roč. 8, jun. (2021), č. článku 670010. ISSN 2296-8016. E-ISSN 2296-8016
Institutional support: RVO:68378041
Keywords : polycaprolactone * hyaluronic acid * vitamin E * nanofibers * oral soft * tissues regeneration * gingival fibroblasts * collagen turnover
OECD category: Biomaterials (as related to medical implants, devices, sensors)
Impact factor: 3.985, year: 2021
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fmats.2021.670010/full

Aim: The regeneration of small periodontal defects has been considered an important divide and challenging issue for dental practitioners. The aim of this preliminary in vitro study was to analyze the effects of polycaprolactone (PCL) nanofibers enriched with hyaluronic acid and vitamin E vs. nude nanofibers on gingival fibroblasts activity, an innovative graft for periodontal soft tissue regeneration purposes.

Methods: Nanofibers were produced in PCL (NF) or PCL enriched with hyaluronic acid and vitamin E (NFE) by electrospinning technique. NF and NFE were stereologically and morphologically characterized by scanning electron microscope (SEM), and composition was analyzed by infrared spectroscopy. Human fibroblasts were obtained from one gingival tissue fragment (HGF) and then seeded on NF, NFE, and plastic (CT). Cell adhesion and morphology were evaluated using SEM at 24 h and cell viability after 24, 48, and 72 h by alamarBlue (R) assay. Gene expression for COL-I, LH2b, TIMP-1, PAX, and VNC was analyzed by real-time RT-PCR in samples run in triplicate and GAPDH was used as housekeeping gene. Slot blot analysis was performed and immunoreactive bands were revealed for MMP-1 and COL-I. YAP and p-YAP were analyzed by Western blot and membranes were reprobed by alpha-tubulin. Statistical analysis was performed.

Results: IR spectrum revealed the presence of PCL in NF and PCL and vitamin E and hyaluronic acid in NFE. At 24 h, HGF adhered on NF and NFE conserving fibroblast like morphology. At 72 h from seeding, statistically significant differences were found in proliferation of HGF cultured on NF compared to NFE. Expression of genes (LH2b, TIMP-1, and MMP-1) and proteins (COL-I) related to collagen turnover revealed a reduction of COL-1 secretion in cells cultured on NF and NFE compared to CT, however, NFE stimulated cross-linked collagen deposition. Mechanosensor genes (PAX, VNC, and YAP) were upregulated in HGF on NF while they were decreased in cells grown on NFE.

Conclusion: Preliminary data suggest that PCL-enriched nanofibers could represent a support to induce HGF proliferation, adhesion, collagen cross-linking, and to reduce collagen degradation, therefore favoring collagen deposition in gingival connective tissue.
Permanent Link: https://hdl.handle.net/11104/0333334