Quantitative mapping of mercury and selenium in mushroom fruit bodies with laser ablation–inductively coupled plasma‑mass spectrometry

0559944 - GLÚ 2023 RIV DE eng J - Journal Article
Braeuer, S. - van Helden, T. - van Acker, T. - Leroux, O. - Van Der Straeten, D. - Verbeken, A. - Borovička, Jan - Vanhaecke, F.
Quantitative mapping of mercury and selenium in mushroom fruit bodies with laser ablation–inductively coupled plasma‑mass spectrometry.
Analytical and Bioanalytical Chemistry. Roč. 414, č. 25 (2022), s. 7517-7530. ISSN 1618-2642. E-ISSN 1618-2650
Institutional support: RVO:67985831
Keywords : Bioimaging * Heavy metal * Trace element * Macrofungi * LA-ICP-MS * Quantification
OECD category: Analytical chemistry
Impact factor: 4.3, year: 2022
Method of publishing: Open access
https://link.springer.com/article/10.1007/s00216-022-04240-y#citeas

This work describes the development of a novel method for quantitative mapping of Hg and Se in mushroom fruit body tissues with laser ablation coupled to inductively coupled plasma-mass spectrometry (LA-ICP-MS). Different parameters of the protocol for preparation of the standards used for quantification via external calibration were assessed, e.g., the dissolution temperature of gelatin standards and the addition of chitosan and ʟ-cysteine as additives to the gelatin-based calibration droplets to better match the sample matrix. While chitosan was not suited for this purpose, the presence of ʟ-cysteine considerably improved the figures of merit of the calibration, leading to limits of detection of 0.006 and 0.3 µg g⁻¹ for Hg and Se, respectively, at a pixel size of 20 × 20 µm. Further, an in-house reference material, ideally suited for the validation of the method for application to mushroom samples, was successfully prepared from a paste of Boletus edulisi. The newly developed method was used to investigate the distribution of Hg and Se in tissue sections of five porcini mushroom individuals of three different species (Boletus edulis, Boletus aereus, and Boletus pinophilus) and one sample of a parasol mushroom (Macrolepiota procera). For one sample, additional areas were ablated at higher spatial resolution, with a laser spot size down to 5 µm, which allows a detailed investigation of the spatial distribution of Hg and Se in mushrooms.
Permanent Link: https://hdl.handle.net/11104/0336092