Clustered DNA Damage Formation in Human Cells after Exposure to Low- and Intermediate-Energy Accelerated Heavy Ions

0559818 - BFÚ 2023 RIV RU eng J - Journal Article
Zadneprianetc, M. - Boreyko, A. - Jezkova, L. - Falk, Martin - Ryabchenko, A. - Hramco, T. - Krupnova, M. - Kulíková, D. - Pavlova, A. A. - Shamina, D. - Smirnova, E. - Krasavin, E.
Clustered DNA Damage Formation in Human Cells after Exposure to Low- and Intermediate-Energy Accelerated Heavy Ions.
Physics of Particles and Nuclei Letters. Roč. 19, č. 4 (2022), s. 440-450. ISSN 1547-4771
Institutional support: RVO:68081707
Keywords : clustered DNA damage and repair * radiation-induced foci (RIF) * heavy ions * protons * y-rays * foci complexity and structure
OECD category: Oncology
Impact factor: 0.5, year: 2022
Method of publishing: Limited access
https://link.springer.com/article/10.1134/S1547477122040227

Based on the differentiated analysis of individual radiation-induced foci in three-dimensional images reconstructing the entire volume of the cell nucleus, a detailed analysis of the structure of complex DNA damage clusters in accelerated ion tracks is carried out, and the differences in damage cluster morphology are investigated. It has been found that after exposure to accelerated heavy ions of low and intermediate energies, unlike gamma-rays, complex clusters are formed, including up to six and more individual foci. The obtained results showed that the structure of clustered DNA damage depend on particle LET. The kinetics of the elimination of radiation-induced foci in cells after heavy ion exposure is shown to be slower than after gamma-irradiation. It is concluded that the delay in kinetics is associated with the nature of the microdistribution of heavy charged particles' energy in genetic structures and the formation of complex DNA double-strand breaks (DSBs), which are difficult to repair. A study of the kinetics of radiation-induced foci formation and elimination after exposure to accelerated boron, nitrogen, and neon ions with different physical characteristics has shown that with a decrease in the particles' energy and an increase in their LET, the effectiveness of DNA DSB repair decreases. It is shown that the structure of radiation-induced foci caused by accelerated ions with different physical characteristics is also different. Neon ions with a high density of delta-electrons in the track induce larger and more complex foci clusters.
Permanent Link: https://hdl.handle.net/11104/0340507