Anionically functionalized glycogen encapsulates melittin by multivalent interaction

0559765 - ÚMCH 2023 RIV US eng J - Journal Article
Zhukouskaya, Hanna - Blanco, P. M. - Černochová, Zulfiya - Čtveráčková, Lucie - Staňo, R. - Pavlova, Ewa - Vetrík, Miroslav - Černoch, Peter - Filipová, Marcela - Šlouf, Miroslav - Štěpánek, M. - Hrubý, Martin - Košovan, P. - Pánek, Jiří
Anionically functionalized glycogen encapsulates melittin by multivalent interaction.
Biomacromolecules. Roč. 23, č. 8 (2022), s. 3371-3382. ISSN 1525-7797. E-ISSN 1526-4602
R&D Projects: GA ČR(CZ) GA19-10429S; GA ČR(CZ) GA21-04166S
Research Infrastructure: e-INFRA CZ - 90140
Institutional support: RVO:61389013
Keywords : melitin * glycogen * polyelectrolyte
OECD category: Polymer science
Impact factor: 6.2, year: 2022
Method of publishing: Limited access
https://pubs.acs.org/doi/10.1021/acs.biomac.2c00400

We developed acid-functionalized glycogen conjugates as supramolecular carriers for efficient encapsulation and inhibition of a model cationic peptide melittin─the main component of honeybee venom. For this purpose, we synthesized and characterized a set of glycogens, functionalized to various degrees by several different acid groups. These conjugates encapsulate melittin up to a certain threshold amount, beyond which they precipitate. Computer simulations showed that sufficiently functionalized conjugates electrostatically attract melittin, resulting in its efficient encapsulation in a broad pH range around the physiological pH. Hemolytic assays confirmed in vitro that the effective inhibition of melittin’s hemolytic activity occurs for highly functionalized samples, whereas no inhibition is observed when using low-functionalized conjugates. It can be concluded that functional glycogens are promising carriers for cationic molecular cargos or antidotes against animal venoms under conditions, in which suitable properties such as biodegradability and biocompatibility are crucial.
Permanent Link: https://hdl.handle.net/11104/0334942