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Focal Adhesion Protein Vinculin Is Required for Proper Meiotic Progression during Mouse Spermatogenesis

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    0559436 - ÚMG 2023 RIV CH eng J - Journal Article
    Petrusová, Jana - Havalda, Robert - Flachs, Petr - Venit, Tomáš - Darášová, Alžběta - Hůlková, Lenka - Sztacho, Martin - Hozák, Pavel
    Focal Adhesion Protein Vinculin Is Required for Proper Meiotic Progression during Mouse Spermatogenesis.
    Cells. Roč. 11, č. 13 (2022), č. článku 2013. E-ISSN 2073-4409
    R&D Projects: GA ČR GA19-05608S; GA ČR(CZ) GA18-19714S; GA MŠMT LTC19048; GA MŠMT LTC20024; GA MŠMT(CZ) ED1.1.00/02.0109; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) EF18_046/0016045
    Grant - others:AV ČR(CZ) JSPS-20-06
    Program: Bilaterální spolupráce
    Institutional support: RVO:68378050
    Keywords : vinculin * spermatogenesis * centromere synapsis * kinetochore * ubiquitin-proteasome system * fertility
    OECD category: Cell biology
    Impact factor: 6, year: 2022
    Method of publishing: Open access
    https://www.mdpi.com/2073-4409/11/13/2013

    The focal adhesion protein Vinculin (VCL) is ascribed to various cytoplasmic functions, however, its nuclear role has so far been ambiguous. We observed that VCL localizes to the nuclei of mouse primary spermatocytes undergoing first meiotic division. Specifically, VCL localizes along the meiosis-specific structure synaptonemal complex (SC) during prophase I and the centromeric regions, where it remains until metaphase I. To study the role of VCL in meiotic division, we prepared a conditional knock-out mouse (VCLcKO). We found that the VCLcKO male mice were semi-fertile, with a decreased number of offspring compared to wild-type animals. This study of events in late prophase I indicated premature splitting of homologous chromosomes, accompanied by an untimely loss of SCP1. This caused erroneous kinetochore formation, followed by failure of the meiotic spindle assembly and metaphase I arrest. To assess the mechanism of VCL involvement in meiosis, we searched for its possible interacting partners. A mass spectrometry approach identified several putative interactors which belong to the ubiquitin-proteasome pathway (UPS). The depletion of VLC leads to the dysregulation of a key subunit of the proteasome complex in the meiotic nuclei and an altered nuclear SUMOylation level. Taken together, we show for the first time the presence of VCL in the nucleus of spermatocytes and its involvement in proper meiotic progress. It also suggests the direction for future studies regarding the role of VCL in spermatogenesis through regulation of UPS.
    Permanent Link: https://hdl.handle.net/11104/0332876

     
     
Number of the records: 1  

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