Biosensor for rapid detection of SARS-CoV-2 in real-world samples

0558950 - FZÚ 2023 RIV US eng C - Conference Paper (international conference)
Forinová, Michala - Pilipenco, Alina - Víšová, Ivana - Kunčák, Jakub - Lynn, Nicholas Scott - Yudin, Petr - Dostálek, Jakub - Hönig, Václav - Palus, Martin - Mašková, H. - Dyčka, F. - Štěrba, J. - Vrabcová, Markéta - Arnoštová, Judita - Spasovová, Monika - Tung, C.-P. - Yang, A.-S. - Dejneka, Alexandr - Vaisocherová-Lísalová, Hana
Biosensor for rapid detection of SARS-CoV-2 in real-world samples.
IEEE Sensors. In: IEEE SENSORS 2021. New York: IEEE, 2021 - (McShane, M.), (2021), s. 1-4. ISBN 978-1-7281-9501-8. ISSN 1930-0395.
[IEEE Sensors Conference /20./. Sydney (AU), 31.10.2021-03.11.2021]
R&D Projects: GA ČR(CZ) GA21-19779S
Grant - others:AV ČR(CZ) LQ100101902
Program: Prémie Lumina quaeruntur
Institutional support: RVO:68378271 ; RVO:60077344
Keywords : biosensor * SARS-CoV-2 * polymer brush * QCM * A-QCM * rapid detection * antifouling
OECD category: Biophysics; Biophysics (BC-A)

The current COVID-19 pandemic has become a worldwide problem with more than 169 million people infected by May 2021. Here we demonstrate a unique technology, based on the quartz crystal microbalance method, for the rapid detection of SARS-CoV-2. This biosensor fulfils all of the many requirements for the rapid detection of SARS-CoV-2 in complex samples. This is achieved by a tailored antifouling surface post-modified with antibodies against SARS-CoV-2 nucleocapsid protein (N). The A-QCM profits from absence of sample pre-treatment and utilizes the natural properties of N protein, which forms complexes with vRNA. Thanks to this, the clinically relevant LOD of 6.7×103 PFU/mL was reached using one-step detection assay. The A-QCM biosensor was also validated with clinical samples (i.e. nasopharyngeal swabs) with full agreement with qRT-PCR.
Permanent Link: https://hdl.handle.net/11104/0332437