Regulation of Phenolic Compound Production by Light Varying in Spectral Quality and Total Irradiance

0558870 - ÚVGZ 2023 RIV CH eng J - Journal Article
Pech, R. - Volna, A. - Hunt, L. - Bartas, M. - Cerven, J. - Pečinka, P. - Špunda, Vladimír - Nezval, J.
Regulation of Phenolic Compound Production by Light Varying in Spectral Quality and Total Irradiance.
International Journal of Molecular Sciences. Roč. 23, č. 12 (2022), č. článku 6533. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) EF16_019/0000797
Institutional support: RVO:86652079
Keywords : barley hordeum-vulgare * gene-expression * green light * red-light * anthocyanin biosynthesis * hydroxycinnamic acids * antioxidant activity * mediated regulation * oxidative stress * functional roles * antioxidants * flavonoids * hplc * miRNA * photoprotection * secondary metabolism * spectral quality of light * spring barley (Hordeum vulgare) * transcriptomics * UV tolerance
OECD category: Biochemistry and molecular biology
Impact factor: 5.6, year: 2022
Method of publishing: Limited access
https://www.mdpi.com/1422-0067/23/12/6533

Photosynthetically active radiation (PAR) is an important environmental cue inducing the production of many secondary metabolites involved in plant oxidative stress avoidance and tolerance. To examine the complex role of PAR irradiance and specific spectral components on the accumulation of phenolic compounds (PheCs), we acclimated spring barley (Hordeum vulgare) to different spectral qualities (white, blue, green, red) at three irradiances (100, 200, 400 mu mol m(-2) s(-1)). We confirmed that blue light irradiance is essential for the accumulation of PheCs in secondary barley leaves (in UV-lacking conditions), which underpins the importance of photoreceptor signals (especially cryptochrome). Increasing blue light irradiance most effectively induced the accumulation of B-dihydroxylated flavonoids, probably due to the significantly enhanced expression of the F3 ' H gene. These changes in PheC metabolism led to a steeper increase in antioxidant activity than epidermal UV-A shielding in leaf extracts containing PheCs. In addition, we examined the possible role of miRNAs in the complex regulation of gene expression related to PheC biosynthesis.
Permanent Link: http://hdl.handle.net/11104/0332369