Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA Gm15551

0558861 - ÚMG 2023 RIV CH eng J - Journal Article
Engelhardt, C. - Huang, C. - Khani, S. - Kašpárek, Petr - Procházka, Jan - Rozman, Jan - Reguera, David Pajuelo - Sedláček, Radislav - Kornfeld, J.W.
Comprehensive Transcriptional Profiling and Mouse Phenotyping Reveals Dispensable Role for Adipose Tissue Selective Long Noncoding RNA Gm15551.
Non-coding RNA. Roč. 8, č. 3 (2022), č. článku 32. E-ISSN 2311-553X
R&D Projects: GA MŠMT(CZ) LM2018126
Institutional support: RVO:68378050
Keywords : long noncoding RNAs * brown adipose tissue * adipose tissue remodelling
OECD category: Biochemistry and molecular biology
Impact factor: 4.3, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2311-553X/8/3/32

Cold and nutrient-activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via the uncoupled respiration and secretion of endocrine factors, thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine-tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to interscapular brown adipose tissue (iBAT) function in a high fat diet (HFD) and cold stressed mice. We focused on Gm15551, which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis, and downregulated by beta-adrenergic activation in mature adipocytes. Although we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.
Permanent Link: https://hdl.handle.net/11104/0332898