Specific anion effect on properties of HRV 3C protease

0558421 - MBÚ 2023 RIV NL eng J - Journal Article
Dusekova, E. - Berta, M. - Sedlakova, D. - Řeha, D. - Dzurillova, V. - Shaposhnikova, Anastasiia - Fadaei, Fatemeh - Tomková, M. - Minofar, B. - Sedlák, E.
Specific anion effect on properties of HRV 3C protease.
Biophysical Chemistry. Roč. 287, AUG 2022 (2022), č. článku 106825. ISSN 0301-4622. E-ISSN 1873-4200
Institutional support: RVO:61388971
Keywords : hofmeister series * thermal-stability * enzyme-activity * conformational flexibility * initial configurations * substrate-specificity * molecular-dynamics * nadh oxidase * activation * ions * Hofmeister effect * Enzyme activation * Cysteine protease
OECD category: Biophysics
Impact factor: 3.8, year: 2022
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0301462222000679?via%3Dihub

Specific salts effect is intensively studied from the prospective of modification of different physico-chemical properties of biomacromolecules. Limited knowledge of the specific salts effect on enzymes led us to address the influence of five sodium anions: sulfate, phosphate, chloride, bromide, and perchlorate, on catalytic and conformational properties of human rhinovirus-14 (HRV) 3C protease. The enzyme conformation was monitored by circular dichroism spectrum (CD) and by tyrosines fluorescence. Stability and flexibility of the enzyme have been analyzed by CD in the far-UV region, differential scanning calorimetry and molecular dynamics simulations, respectively. We showed significant influence of the anions on the enzyme properties in accordance with the Hofmeister effect. The HRV 3C protease in the presence of kosmotropic anions, in contrast with chaotropic anions, exhibits increased stability, rigidity. Correlations of stabilization effect of anions on the enzyme with their charge density and the rate constant of the enzyme with the viscosity B-coefficients of anions suggest direct interaction of the anions with HRV 3C protease. The role of stabilization and decreased fluctuation of the polypeptide chain of HRV 3C protease on its activation in the presence of kosmotropic anions is discussed within the frame of the macromolecular rate theory.
Permanent Link: https://hdl.handle.net/11104/0332425