Non-enzymatic electrochemical determination of cholesterol in dairy products on boron-doped diamond electrode

0558154 - ÚOCHB 2023 RIV NL eng J - Journal Article
Benešová, L. - Klouda, J. - Bláhová, E. - Nesměrák, K. - Kočovský, Pavel - Nádvorníková, J. - Barták, P. - Skopalová, J. - Schwarzová-Pecková, K.
Non-enzymatic electrochemical determination of cholesterol in dairy products on boron-doped diamond electrode.
Food Chemistry. Roč. 393, November (2022), č. článku 133278. ISSN 0308-8146. E-ISSN 1873-7072
Institutional support: RVO:61388963
Keywords : boron-doped diamond * cholesterol * dairy products * differential pulse voltammetry * GC-MS * oxidation
OECD category: Analytical chemistry
Impact factor: 8.8, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.foodchem.2022.133278

Determination of cholesterol in food matrices is essential for quality control concerning the health of consumers. Herein, a simple electrochemical approach for cholesterol quantitation in dairy products is evaluated. The newly developed differential pulse voltammetric method using acetonitrile-perchloric acid mixture as a supporting electrolyte is statistically compared to GC–MS and HPLC-UV. Oxidation signals of cholesterol at +1.5 V and +1.4 V (vs. Ag/AgNO3 in acetonitrile) provide detection limits of 4.9 µM and 6.1 µM on boron-doped diamond and glassy carbon electrodes, respectively. A simple liquid–liquid extraction procedure from dairy products into hexane resulted in a recovery rate of (74.8 ± 3.8)%. The method provides results in close agreement (at a 95% confidence level) with GC–MS, while HPLC-UV resulted in a significant difference in estimated cholesterol concentrations for all samples. This newly developed method is a simpler, faster and cheaper alternative to instrumentally demanding MS-based methods and clearly outperforms HPLC-UV.
Permanent Link: http://hdl.handle.net/11104/0331951