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High plasmidome diversity of extended-spectrum beta-lactam-resistant Escherichia coli isolates collected during one year in one community hospital

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    0557996 - MBÚ 2023 RIV US eng J - Journal Article
    Neffe, L. - Abendroth, L. - Bautsch, W. - Haeussler, S. - Tomasch, Jurgen
    High plasmidome diversity of extended-spectrum beta-lactam-resistant Escherichia coli isolates collected during one year in one community hospital.
    Genomics. Roč. 114, č. 3 (2022), č. článku 110368. ISSN 0888-7543. E-ISSN 1089-8646
    Institutional support: RVO:61388971
    Keywords : sequence type 131 * dissemination * gene * mobility * c1-m27 * spread * clone * Escherichia coli * Plasmids * Horizontal gene transfer * Antimicrobial resistance * Sequencing
    OECD category: Microbiology
    Impact factor: 4.4, year: 2022
    Method of publishing: Open access
    https://www.sciencedirect.com/science/article/pii/S0888754322001136?via%3Dihub

    Plasmid-encoded antibiotic resistance encompasses many classes of currently used antibiotics. In globally distributed Escherichia coli lineages plasmids, which spread via horizontal gene transfer, are responsible for the dissemination of genes encoding extended-spectrum 13-lactamases (ESBL). In this study, we combined 2nd and 3rd generation sequencing techniques to reconstruct the plasmidome of overall 97 clinical ESBL-E. coli isolates. Our results highlight the enormous plasmid diversity in respect to size, replicon-type and genetic content. Furthermore, we emphasize the diverse plasmid distribution patterns among the clinical isolates and the high intra-and extracellular mobility potential of resistance conferring genes. While the majority of resistance conferring genes were located on large plasmids of known replicon type, small cryptic plasmids seem to be underestimated resistance gene vectors. Our results contribute to a better understanding of the dissemination of resistance-conferring genes through horizontal gene transfer as well as clonal spread.
    Permanent Link: https://hdl.handle.net/11104/0332690

     
     
Number of the records: 1  

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