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Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura

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    0557799 - MBÚ 2023 RIV GB eng J - Journal Article
    Pietsch, G. - Gazis, R. - Klingeman, W. - Huff, M. - Staton, M. - Kolařík, Miroslav - Hadziabdic, D.
    Characterization and microsatellite marker development for a common bark and ambrosia beetle associate, Geosmithia obscura.
    MicrobiologyOpen. Roč. 11, č. 3 (2022), č. článku e1286. ISSN 2045-8827. E-ISSN 2045-8827
    Institutional research plan: CEZ:AV0Z50200510
    Keywords : thousand cankers disease * causal agent * host-range * sp nov. * genome * diversity * morbida * walnut * differentiation * identification * beetle-fungus symbiosis * Bionectriaceae * cross-amplification * detection * microsatellite markers
    OECD category: Microbiology
    Impact factor: 3.904, year: 2021
    Method of publishing: Open access
    https://www.frontiersin.org/articles/10.3389/fmicb.2022.869661/full

    Symbioses between Geosmithia fungi and wood-boring and bark beetles seldom result in disease induction within the plant host. Yet, exceptions exist such as Geosmithia morbida, the causal agent of Thousand Cankers Disease (TCD) of walnuts and wingnuts, and Geosmithia sp. 41, the causal agent of Foamy Bark Canker disease of oaks. Isolates of G. obscura were recovered from black walnut trees in eastern Tennessee and at least one isolate induced cankers following artificial inoculation. Due to the putative pathogenicity and lack of recovery of G. obscura from natural lesions, a molecular diagnostic screening tool was developed using microsatellite markers mined from the G. obscura genome. A total of 3256 candidate microsatellite markers were identified (2236, 789, 137 di-, tri-, and tetranucleotide motifs, respectively), with 2011, 703, 101 di-, tri-, and tetranucleotide motifs, respectively, containing markers with primers. From these, 75 microsatellite markers were randomly selected, screened, and optimized, resulting in 28 polymorphic markers that yielded single, consistently recovered bands, which were used in downstream analyses. Five of these microsatellite markers were found to be specific to G. obscura and did not cross-amplify into other, closely related species. Although the remaining tested markers could be useful, they cross-amplified within different Geosmithia species, making them not reliable for G. obscura detection. Five novel microsatellite markers (GOBS9, GOBS10, GOBS41, GOBS43, and GOBS50) were developed based on the G. obscura genome. These species-specific microsatellite markers are available as a tool for use in molecular diagnostics and can assist future surveillance studies.
    Permanent Link: http://hdl.handle.net/11104/0331696

     
     
Number of the records: 1