The complete mitochondrial genome of carnivorous Genlisea tuberosa (Lentibulariaceae): Structure and evolutionary aspects

0557784 - BÚ 2023 RIV NL eng J - Journal Article
Matos, R. - Silva, S. - Plachno, B. - Adamec, Lubomír - Michael, T. - Varani, A. - Miranda, V.
The complete mitochondrial genome of carnivorous Genlisea tuberosa (Lentibulariaceae): Structure and evolutionary aspects.
Gene. Roč. 824, MAY 25 2022 (2022), č. článku 146391. ISSN 0378-1119. E-ISSN 1879-0038
Institutional support: RVO:67985939
Keywords : mitochondrial genes * chloroplast genes * carnivorous lifestyle
OECD category: Biochemistry and molecular biology
Impact factor: 3.5, year: 2022
Method of publishing: Limited access
https://doi.org/10.1016/j.gene.2022.146391

In this study, we sequenced and characterized the mitochondrial genome of the tuberous carnivorous plant Genlisea tuberosa, being the first of its genus to be sequenced. The genome comprises 729,765 bp, encoding 80 identified genes of which 36 are protein coding, 40 tRNA, four rRNA genes, and three pseudogenes. An intronic region from the cox1 gene was identified that encodes an endonuclease enzyme that is present in the other sequenced species of Lentibulariaceae. Chloroplast genes (pseudogene and complete) inserted in the MT genome were identified, showing possible horizontal transfer between organelles. In addition, 50 pairs of long repeats from 94 to 274 bp are present, possibly playing an important role in the maintenance of the MT genome. Phylogenetic analysis carried out with 34 coding mitochondrial genes corroborated the positioning of the species listed here within the family. The molecular dynamism in the mitogenome (e.g. the loss or pseudogenization of genes, insertion of foreign genes, the long repeats as well as accumulated mutations) may be reflections of the carnivorous lifestyle where a significant part of cellular energy was shifted for the adaptation of leaves into traps molding the mitochondrial DNA. The sequence and annotation of G. tuberosa’s MT will be useful for further studies and serve as a model for evolutionary and taxonomic clarifications of the group as well as improving our comprehension of MT evolution.
Permanent Link: https://hdl.handle.net/11104/0332970