The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations

0557670 - ÚFCH JH 2023 RIV CH eng J - Journal Article
Schicht, M. - Riedlová, Kamila - Kukulka, M. - Li, W. - Scheer, A. - Garreis, F. - Jacobi, Ch. - Paulsen, F. - Cwiklik, Lukasz - Bräuer, L.
The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations.
International Journal of Molecular Sciences. Roč. 23, č. 10 (2022), č. článku 5783. E-ISSN 1422-0067
R&D Projects: GA ČR(CZ) GA21-19854S
Institutional support: RVO:61388955
Keywords : tear film * surfactant protein * surface tension
OECD category: Physical chemistry
Impact factor: 5.6, year: 2022
Method of publishing: Open access

The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film. View Full-Text
Permanent Link: http://hdl.handle.net/11104/0331578