Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales

0557609 - MBÚ 2023 RIV GB eng J - Journal Article
Tomasch, Jurgen - Ringel, V. - Wang, H. - Freese, H. - Bartling, P. - Brinkmann, H. - Vollmers, J. - Jarek, M. - Wagner-Doebler, I. - Petersen, J.
Fatal affairs - conjugational transfer of a dinoflagellate- killing plasmid between marine Rhodobacterales.
Microbial Genomics. Roč. 8, č. 3 (2022), č. článku 000787. ISSN 2057-5858. E-ISSN 2057-5858
Institutional support: RVO:61388971
Keywords : phaeobacter-gallaeciensis * dinoroseobacter-shibae * tropodithietic acid * gen. nov. * roseobacter * genome * phytoplankton * adaptation * bacteria * agent * bacteria-algae interaction * conjugation * horizontal gene transfer * Roseobacter
OECD category: Microbiology
Impact factor: 3.9, year: 2022
Method of publishing: Open access
https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000787

The roseobacter group of marine bacteria is characterized by a mosaic distribution of ecologically important phenotypes. These are often encoded on mobile extrachromosomal replicons. So far, conjugation had only been experimentally proven between the two model organisms Phaeobacter inhibens and Dinoroseobacter shibae. Here, we show that two large natural RepABCtype plasmids from D. shibae can be transferred into representatives of all known major Rhodobacterales lineages. Complete genome sequencing of the newly established Phaeobacter inhibens transconjugants confirmed their genomic integrity. The conjugated plasmids were stably maintained as single copy number replicons in the genuine as well as the new host. Co- cultivation of Phaeobacter inhibens and the transconjugants with the dinoflagellate Prorocentrum minimum demonstrated that Phaeobacter inhibens is a probiotic strain that improves the yield and stability of the dinoflagellate culture. The transconjugant carrying the 191 kb plasmid, but not the 126 kb sister plasmid, killed the dinoflagellate in co- culture.
Permanent Link: http://hdl.handle.net/11104/0331597