Cobalt Bis-Dicarbollide Enhances Antibiotics Action towards Staphylococcus epidermidis Planktonic Growth Due to Cell Envelopes Disruption

0557551 - ÚOCHB 2023 RIV CH eng J - Journal Article
Vaňková, E. - Lokočová, K. - Kašparová, P. - Hadravová, Romana - Křížová, I. - Mat’átková, O. - Masák, J. - Šícha, V.
Cobalt Bis-Dicarbollide Enhances Antibiotics Action towards Staphylococcus epidermidis Planktonic Growth Due to Cell Envelopes Disruption.
Pharmaceuticals. Roč. 15, č. 5 (2022), č. článku 534. E-ISSN 1424-8247
R&D Projects: GA MŠMT(CZ) EF16_019/0000729
Institutional support: RVO:61388963
Keywords : additive effect * antibiotics * antimicrobial activity * carborane * erythromycin * gram-positive bacterium * metallacarboranes * synergistic effect * tetracycline * vancomycin
OECD category: Microbiology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://doi.org/10.3390/ph15050534

The emergence of antibiotic resistance in opportunistic pathogens represents a huge problem, the solution for which may be a treatment with a combination of multiple antimicrobial agents. Sodium salt of cobalt bis-dicarbollide (COSAN.Na) is one of the very stable, low-toxic, amphiphilic boron-rich sandwich complex heteroboranes. This compound has a wide range of potential applications in the biological sciences due to its antitumor, anti-HIV-1, antimicrobial and antibiofilm activity. Our study confirmed the ability of COSAN.Na (in the concentration range 0.2–2.48 µg/mL) to enhance tetracycline, erythromycin, and vancomycin action towards Staphylococcus epidermidis planktonic growth with an additive or synergistic effect (e.g., the combination of 1.24 µg/mL COSAN.Na and 6.5 µg/mL TET). The effective inhibitory concentration of antibiotics was reduced up to tenfold most efficiently in the case of tetracycline (from 65 to 6.5 µg/mL). In addition, strong effect of COSAN.Na on disruption of the cell envelopes was determined using propidium iodide uptake measurement and further confirmed by transmission electron microscopy. The combination of amphiphilic COSAN.Na with antibiotics can therefore be considered a promising way to overcome antibiotic resistance in Gram-positive cocci.
Permanent Link: http://hdl.handle.net/11104/0331517