The agonistic bioanalytical equivalent concentration: A novel tool for assessing the endocrine activity of environmental mixtures

0557456 - MBÚ 2023 RIV NL eng J - Journal Article
Ezechiáš, Martin
The agonistic bioanalytical equivalent concentration: A novel tool for assessing the endocrine activity of environmental mixtures.
Environmental Toxicology and Pharmacology. Roč. 89, JAN 2022 (2022), č. článku 103781. ISSN 1382-6689. E-ISSN 1872-7077
R&D Projects: GA ČR(CZ) GJ20-14318Y
Institutional support: RVO:61388971
Keywords : full logistic model * estrogenic activity * chemical-analysis * water * identification * surface * assays * Endocrine disruptors * Concentration addition * Mixture toxicity * Competitive antagonist * Bioanalytical equivalent concentration * Effect-based methods
OECD category: Environmental sciences (social aspects to be 5.7)
Impact factor: 4.3, year: 2022
Method of publishing: Limited access
https://www.sciencedirect.com/science/article/pii/S138266892100199X?via%3Dihub

Cell-based bioassays are very sensitive and allow integrative effect screening of the whole environmental sample, which is usually composed of a mixture of agonists and antagonists. Measured toxicity is usually expressed as a bioanalytical equivalent concentration. So far, it is not possible to distinguish which part of this value is caused by the agonists and which by the antagonists. In this article, we present a simple method to analyze the dose response curve of a mixture and to determine an agonistic bioanalytical equivalent concentration: a concentration of a reference chemical that would elicit the same effect as do only agonists in an unknown mixture. The method has been validated using several artificially prepared mixtures of agonists and competitive antagonists measured in a recombinant yeast assay. No difference was observed between the calculated equivalent concentrations and the used concentrations of the agonist in the mixture.
Permanent Link: http://hdl.handle.net/11104/0331562