Phosphorus-containing polymeric zwitterion: a pioneering bioresponsive probe for 31P-magnetic resonance imaging

0557336 - ÚMCH 2023 RIV DE eng J - Journal Article
Kracíková, Lucie - Ziolkowska, N. - Androvič, Ladislav - Klimánková, Iveta - Červený, D. - Vít, M. - Pompach, Petr - Konefal, Rafal - Janoušková, Olga - Hrubý, Martin - Jirák, D. - Laga, Richard
Phosphorus-containing polymeric zwitterion: a pioneering bioresponsive probe for 31P-magnetic resonance imaging.
Macromolecular Bioscience. Roč. 22, č. 5 (2022), č. článku 2100523. ISSN 1616-5187. E-ISSN 1616-5195
R&D Projects: GA MZd(CZ) NU20-08-00095; GA ČR(CZ) GA19-08176S; GA ČR(CZ) GA21-01090S; GA MŠMT(CZ) LTAUSA18173; GA MŠMT(CZ) LM2018127
Research Infrastructure: CIISB II - 90127
Institutional support: RVO:61389013 ; RVO:86652036
Keywords : 31P-MRI * MRI probes * polymer zwitterions
OECD category: Polymer science; Analytical chemistry (BTO-N)
Impact factor: 4.6, year: 2022
Method of publishing: Limited access
https://onlinelibrary.wiley.com/doi/10.1002/mabi.202100523

31P-magnetic resonance (MR) is an important diagnostic technique currently used for tissue metabolites assessing, but it also has great potential for visualizing the internal body structures. However, due to the low physiological level of phosphorus-containing biomolecules, precise imaging requires the administration of an exogenous probe. Herein, this work describes the synthesis and MR characterization of a pioneering metal-free 31P-MR probe based on phosphorus-containing polymeric zwitterion. The developed probe (pTMPC) is a well-defined water-soluble macromolecule characterized by a high content of naturally rare phosphorothioate groups providing a high-intensity 31P-MR signal clearly distinguishable from biological background both in vitro and in vitro. In addition, pTMPC can serve as a sensitive 31P-MR sensor of pathological conditions in vivo because it undergoes oxidation-induced structural changes in the presence of reactive oxygen species (ROS). Add to this the favorable 1H and 31P T1/T2 relaxation times and biocompatibility, pTMPC represents a conceptually new diagnostic, whose discovery opens up new possibilities in the field of 31P-MR spectroscopy and imaging.

Permanent Link: https://hdl.handle.net/11104/0334711