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Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides
- 1.0557244 - MBÚ 2023 RIV CH eng J - Journal Article
Nekvasilová, Pavlína - Hovorková, Michaela - Mészáros, Zuzana - Petrásková, Lucie - Pelantová, Helena - Křen, Vladimír - Slámová, Kristýna - Bojarová, Pavla
Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides.
International Journal of Molecular Sciences. Roč. 23, č. 8 (2022), č. článku 4106. ISSN 1422-0067. E-ISSN 1422-0067
R&D Projects: GA MŠMT(CZ) LTC20072; GA MŠMT(CZ) LTC19035; GA ČR(CZ) GA20-00215S
EU Projects: European Commission(XE) CA18103
Institutional support: RVO:61388971
Keywords : beta-n-acetylhexosaminidase * crystal-structure * galactosidase * expression * lactose * cloning * glycosynthase * principle * gene * enzymatic synthesis * glycosidase * human milk oligosaccharide * mutagenesis
OECD category: Biochemistry and molecular biology
Impact factor: 5.6, year: 2022
Method of publishing: Open access
https://www.mdpi.com/1422-0067/23/8/4106
Enzymatic synthesis is an elegant biocompatible approach to complex compounds such as human milk oligosaccharides (HMOs). These compounds are vital for healthy neonatal development with a positive impact on the immune system. Although HMOs may be prepared by glycosyltransferases, this pathway is often complicated by the high price of sugar nucleotides, stringent substrate specificity, and low enzyme stability. Engineered glycosidases (EC 3.2.1) represent a good synthetic alternative, especially if variations in the substrate structure are desired. Site-directed mutagenesis can improve the synthetic process with higher yields and/or increased reaction selectivity. So far, the synthesis of human milk oligosaccharides by glycosidases has mostly been limited to analytical reactions with mass spectrometry detection. The present work reveals the potential of a library of engineered glycosidases in the preparative synthesis of three tetrasaccharides derived from lacto-N-tetraose (Gal beta 4GlcNAc beta 3Gal beta 4Glc), employing sequential cascade reactions catalyzed by beta 3-N-acetylhexosaminidase BbhI from Bifidobacterium bifidum, beta 4-galactosidase BgaD-B from Bacillus circulans, beta 4-N-acetylgalactosaminidase from Talaromyces flavus, and beta 3-galactosynthase BgaC from B. circulans. The reaction products were isolated and structurally characterized. This work expands the insight into the multi-step catalysis by glycosidases and shows the path to modified derivatives of complex carbohydrates that cannot be prepared by standard glycosyltransferase methods.
Permanent Link: http://hdl.handle.net/11104/0331292
Number of the records: 1