Sterolight as imaging tool to study sterol uptake, trafficking and efflux in living cells

0557101 - ÚMG 2023 RIV GB eng J - Journal Article
Králová, Jarmila - Popr, Martin - Valečka, Jan - Bartůněk, Petr
Sterolight as imaging tool to study sterol uptake, trafficking and efflux in living cells.
Scientific Reports. Roč. 12, č. 1 (2022), č. článku 6264. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA MŠMT(CZ) EF18_046/0016045; GA MŠMT(CZ) LM2018129; GA ČR GA17-02836S; GA MŠMT(CZ) LM2018130
Institutional support: RVO:68378050
Keywords : endoplasmic-reticulum * cellular cholesterol * plasma-membrane * intracellular-transport * lipid rafts * endocytosis * caveolin * dehydroergosterol * internalization * homeostasis
OECD category: Cell biology
Impact factor: 4.6, year: 2022
Method of publishing: Open access
https://www.nature.com/articles/s41598-022-10134-x

Information about cholesterol subcellular localization and transport pathways inside cells is essential for understanding and treatment of cholesterol-related diseases. However, there is a lack of reliable tools to monitor it. This work follows the fate of Sterolight, a BODIPY-labelled sterol, within the cell and demonstrates it as a suitable probe for visualization of sterol/lipid trafficking. Sterolight enters cells through an energy-independent process and knockdown experiments suggest caveolin-1 as its potential cellular carrier. Intracellular transport of Sterolight is a rapid process, and transfer from ER and mitochondria to lysosomes and later to lipid droplets requires the participation of active microtubules, as it can be inhibited by the microtubule disruptor nocodazole. Excess of the probe is actively exported from cells, in addition to being stored in lipid droplets, to re-establish the sterol balance. Efflux occurs through a mechanism requiring energy and may be selectively poisoned with verapamil or blocked in cells with mutated cholesterol transporter NPC1. Sterolight is efficiently transferred within and between different cell populations, making it suitable for monitoring numerous aspects of sterol biology, including the live tracking and visualization of intracellular and intercellular transport.
Permanent Link: http://hdl.handle.net/11104/0331415