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NMR- and MS-Based Untargeted Metabolomic Study of Stool and Serum Samples from Patients with Anorexia Nervosa

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    0556830 - MBÚ 2023 RIV US eng J - Journal Article
    Tomášová, Petra - Procházková, Petra - Roubalová, Radka - Dvořák, Jiří - Tlaskalová-Hogenová, Helena - Čermáková, Martina - Pelantová, Helena - Šedivá, Blanka - Vecka, M. - Papežová, H. - Kuzma, Marek
    NMR- and MS-Based Untargeted Metabolomic Study of Stool and Serum Samples from Patients with Anorexia Nervosa.
    Journal of Proteome Research. Roč. 21, č. 3 (2022), s. 778-787. ISSN 1535-3893. E-ISSN 1535-3907
    R&D Projects: GA MZd(CZ) NV17-28905A; GA MZd(CZ) NV18-01-00040
    Institutional support: RVO:61388971
    Keywords : oxidative stress * association * antioxidant * leptin * anorexia nervosa * metabolomics * mass spectrometry * nuclear magnetic resonance
    OECD category: Microbiology
    Impact factor: 4.4, year: 2022
    Method of publishing: Limited access
    https://pubs.acs.org/doi/10.1021/acs.jproteome.1c00537

    Anorexia nervosa (AN), a pathological restriction of food intake, leads to metabolic dysregulation. We conducted a metabolomics study to reveal changes caused by AN and the effect of hospital realimentation on metabolism. Both stool and serum from patients with AN and healthy controls were analyzed by NMR and MS. Statistical analysis revealed several altered biochemical and anthropometric parameters and 50 changed metabolites, including phospholipids, acylcarnitines, amino acids, derivatives of nicotinic acid, nucleotides, and energy metabolism intermediates. Biochemical and anthropometric parameters were correlated with metabolomic data. Metabolic changes in patients with AN described in our study imply serious system disruption defects, such as the development of inflammation and oxidative stress, changed free thyroxine (fT4) and thyroid-stimulating hormone (TSH) levels, a deficit of vitamins, muscle mass breakdown, and a decrease in ketone bodies as an important source of energy for the brain and heart. Furthermore, our data indicate only a very slight improvement after treatment. However, correlations of metabolomic results with body weight, interleukin 6, tumor necrosis factor alpha, fT4, and TSH might entail better prognoses and treatment effectiveness in patients with better system parameter status. Data sets are deposited in MassIVE: MSV000087713, DOI: 10.25345/C57R7X.
    Permanent Link: http://hdl.handle.net/11104/0331270

     
     
Number of the records: 1  

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