Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds

0556784 - ÚOCHB 2023 RIV DE eng J - Journal Article
Kvaková, Klaudia - Ondra, M. - Schimer, Jiří - Petrík, M. - Nový, Z. - Raabová, Helena - Hajdúch, M. - Cígler, Petr
Visualization of Sentinel Lymph Nodes with Mannosylated Fluorescent Nanodiamonds.
Advanced Functional Materials. Roč. 32, č. 23 (2022), č. článku 2109960. ISSN 1616-301X. E-ISSN 1616-3028
R&D Projects: GA MŠMT EF16_026/0008382; GA ČR(CZ) GA18-17071S; GA MŠMT(CZ) LM2018133
Institutional support: RVO:61388963
Keywords : cancer imaging * fluorescent nanodiamonds * lymph nodes * macrophages * mannose
OECD category: Radiology, nuclear medicine and medical imaging
Impact factor: 19, year: 2022
Method of publishing: Limited access
https://doi.org/10.1002/adfm.202109960

The present study focuses on the development of a sensitive and specific approach for the visualization of sentinel lymph nodes draining the tumor using ultrabright 200-nm fluorescent nanodiamonds (FNDs) equipped with a designed targeting surface architecture. The FNDs with a narrow size distribution are isolated by differential centrifugation, colloidally stabilized with alkyne-functionalized poly(glycerol) and modified with a polyvalent array of mannose (FND-p-Man). In vitro experiments demonstrate an outstanding increase of the particle internalization by the mannose receptor CD206 (MR) and no significant toxicity. MR involvement is confirmed by blocking ligand binding with mannan and a 15.2 mAb (specific anti-MR) in J774A.1 mouse macrophage cell line. In vivo mouse experiments confirm increased retention of FND-p-Man in sentinel lymph nodes of both healthy and B16 melanoma bearing animals. These results suggest that FND-p-Man has potential as a tracer for lymph node visualization in locoregional perioperative cancer diagnostics and as a tool for endoscopic/robotic fluorescence-guided surgery.
Permanent Link: http://hdl.handle.net/11104/0330980