Waking up from a taxonomist's nightmare: emerging structure of Ranunculus section Batrachium (Ranunculaceae) in central Europe based on molecular data and genome sizes

0556366 - BÚ 2023 RIV GB eng J - Journal Article
Koutecký, P. - Prančl, Jan - Košnar, J. - Koutecká, E. - Hanzlíčková, J. - Lučanová, Magdalena - Nagy Nejedlá, Michaela - Kaplan, Zdeněk
Waking up from a taxonomist's nightmare: emerging structure of Ranunculus section Batrachium (Ranunculaceae) in central Europe based on molecular data and genome sizes.
Botanical Journal of the Linnean Society. Roč. 198, č. 4 (2022), s. 417-437. ISSN 0024-4074. E-ISSN 1095-8339
R&D Projects: GA ČR GA17-06825S
Institutional support: RVO:67985939
Keywords : subgenus batrachium * aquatic plant * hybridization * dna * angiosperms * speciation * sequences * phylogeography * populations * polyploidy * flow cytometry * hybridization * internal transcribed spacer (ITS) * introgression * phylogeny * plastid capture * plastid DNA * polyploidy
OECD category: Plant sciences, botany
Impact factor: 2.4, year: 2022
Method of publishing: Limited access
https://doi.org/10.1093/botlinnean/boab063

Ranunculus section Batrachium (water crowfoot) ranks among the most taxonomically challenging aquatic plant groups due to morphological reduction, phenotypic plasticity, polyploidy and reticulate evolution. This study, for the first time in this group, linked morphology, genome size and genetic data (two non-coding regions of plastid DNA and the ITS region of nuclear ribosomal DNA). This extensive data set, including 258 central European populations, enables us to recognize widespread lineages from hybrids and to trace the evolutionary processes underlying the observed diversity. Most of the traditionally recognized species are supported. However, the presence of two morphologically cryptic but genetically well-differentiated lineages was detected within Ranunculus trichophyllus, and three separate lineages of different parentage were confirmed in the Ranunculus penicillatus complex. An allopolyploid origin was revealed in Ranunculus aquatilis, Ranunculus baudotii and in both lineages of R. trichophyllus, for which the parentage has not yet been studied, and allopolyploidy is suspected in all other polyploid taxa except for the triploid cytotype of Ranunculus fluitans, which is most likely autopolyploid. We detected putative F-1 hybrids of seven different parentage combinations, including two involving Ranunculus rionii, representing the first known hybrids of this species. An additional 13 hybrid cytotypes (backcrosses or entailing additional polyploidization) were revealed, c. 20% of Ranunculus peltatus accessions seem to be influenced by introgression detectable only using sequence data. The Batrachium group is remarkable due to the coexistence of well-defined lineages with recently evolved biotypes arising due to hybridization and polyploidization.
Permanent Link: https://hdl.handle.net/11104/0339163