C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress

0555978 - ÚMG 2023 RIV CH eng J - Journal Article
Klebanovych, Anastasiya - Vinopal, Stanislav - Dráberová, Eduarda - Sládková, Vladimíra - Sulimenko, Tetyana - Sulimenko, Vadym - Vosecká, Věra - Macůrek, Libor - Legido, A. - Dráber, Pavel
C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress.
Cells. Roč. 11, č. 3 (2022), č. článku 555. E-ISSN 2073-4409
R&D Projects: GA ČR(CZ) GA18-27197S; GA ČR(CZ) GA19-20716S; GA ČR GA21-30281S; GA MŠMT(CZ) LTAUSA17052; GA MŠMT LTAUSA19118; GA TA ČR TP01010060; GA MŠMT(CZ) LM2018129
Institutional support: RVO:68378050
Keywords : cdk5rap3 * ER stress * gamma-tubulin * microtubule nucleation * ufl1
OECD category: Cell biology
Impact factor: 6, year: 2022
Method of publishing: Open access
https://www.mdpi.com/2073-4409/11/3/555

ER distribution depends on microtubules, and ER homeostasis disturbance activates the unfolded protein response resulting in ER remodeling. CDK5RAP3 (C53) implicated in various signaling pathways interacts with UFM1-protein ligase 1 (UFL1), which mediates the ufmylation of proteins in response to ER stress. Here we find that UFL1 and C53 associate with gamma-tubulin ring complex proteins. Knockout of UFL1 or C53 in human osteosarcoma cells induces ER stress and boosts centrosomal microtubule nucleation accompanied by gamma-tubulin accumulation, microtubule formation, and ER expansion. C53, which is stabilized by UFL1, associates with the centrosome and rescues microtubule nucleation in cells lacking UFL1. Pharmacological induction of ER stress by tunicamycin also leads to increased microtubule nucleation and ER expansion. Furthermore, tunicamycin suppresses the association of C53 with the centrosome. These findings point to a novel mechanism for the relief of ER stress by stimulation of centrosomal microtubule nucleation.
Permanent Link: http://hdl.handle.net/11104/0330898