Number of the records: 1
Sigma regulatory network in Rhodococcus erythropolis CCM2595
- 1.0555955 - MBÚ 2023 RIV NL eng J - Journal Article
Štěpánek, Václav - Dostálová, Hana - Busche, T. - Blumenstein, Jan - Grulich, Michal - Plašil, Lukáš - Rucká, Lenka - Nešvera, Jan - Pátek, Miroslav
Sigma regulatory network in Rhodococcus erythropolis CCM2595.
FEMS Microbiology Letters. Roč. 369, č. 1 (2022), č. článku fnac014. ISSN 0378-1097. E-ISSN 1574-6968
R&D Projects: GA ČR GA18-13254S
Institutional support: RVO:61388971
Keywords : corynebacterium-glutamicum promoters * vitro transcription system * in-vitro * environmental-stress * expression * responses * tools * vivo * Rhodococcus erythropolis * transcriptional regulatory network * sigma factor * in vitro transcription * RNA-seq
OECD category: Microbiology
Impact factor: 2.1, year: 2022
Method of publishing: Limited access
https://academic.oup.com/femsle/article-abstract/369/1/fnac014/6528914?redirectedFrom=fulltext#no-access-message
The aim of this investigation was to discover the promoters that drive expression of the sig genes encoding sigma factors of RNA polymerase in Rhodococcus erythropolis CCM2595 and classify these promoters according to the sigma factors which control their activity. To analyze the regulation of major sigma factors, which control large regulons that also contain genes expressed under exponential growth and non-stressed conditions, we used the R. erythropolis CCM2595 culture, which grew rapidly in minimal medium. The transcriptional start sites (TSSs) of the genes sigA, sigB, sigD, sigE, sigG, sigH, sigJ, and sigK were detected by primary 5 '-end-specific RNA sequencing. The promoters localized upstream of the detected TSSs were defined by their35 and10 elements, which were identical or closely similar to these sequences in the related species Corynebacterium glutamicum and Mycobacterium tuberculosis. Regulation of the promoter activities by different sigma factors was demonstrated by two independent techniques (in vivo and in vitro). All analyzed sig genes encoding the sigma factors with extracytoplasmic function (ECF) were found to be also driven from additional housekeeping promoters. Based on the classification of the sig gene promoters, a model of the basic sigma transcriptional regulatory network in R. erythropolis was designed.
Permanent Link: http://hdl.handle.net/11104/0330485
Number of the records: 1